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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using GTF2B Polyclonal antibody (16467-1-AP)

    WB analysis of K-562 using 16467-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 16467-1-AP (GTF2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using GTF2B Polyclonal antibody (16467-1-AP)

    WB analysis of HepG2 using 16467-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16467-1-AP (GTF2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using GTF2B Polyclonal antibody (16467-1-AP)

    WB analysis of MCF-7 using 16467-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 16467-1-AP (GTF2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using GTF2B Polyclonal antibody (16467-1-AP)

    WB analysis of HeLa using 16467-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16467-1-AP (GTF2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using GTF2B Polyclonal antibody (16467-1-AP)

    IP experiment of K-562 using 16467-1-AP

    IP result of anti-GTF2B (IP:16467-1-AP, 4ug; Detection:16467-1-AP 1:300) with K-562 cells lysate 3200ug.

GTF2B Polyclonal antibody

GTF2B Polyclonal Antibody for WB, IP, ELISA
Cat No. 16467-1-AP
Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

GTF2B, S300 II, TF2B, TFIIB

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inK-562 cells, HepG2 cells, HeLa cells, MCF-7 cells
Positive IP detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

16467-1-AP targets GTF2B in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9547

Product name: Recombinant human GTF2B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-316 aa of BC020597

Sequence: MASTSRLDALPRVTCPNHPDAILVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAASFDEFGNSKYQNRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLILKALETSVDLITTGDFMSRFCSNLCLPKQVQMAATHIARKAVELDLVPGRSPISVAAAAIYMASQASAEKRTQKEIGDIAGVADVTIRQSYRLIYPRAPDLFPTDFKFDTPVDKLPQL

相同性解析による交差性が予測される生物種
Full Name general transcription factor IIB
Calculated molecular weight 316 aa, 35 kDa
Observed molecular weight 30-35 kDa
GenBank accession numberBC020597
Gene Symbol GTF2B
Gene ID (NCBI) 2959
RRIDAB_2878263
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ00403
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IP protocol for GTF2B antibody 16467-1-APDownload protocol
WB protocol for GTF2B antibody 16467-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Combinatorial ubiquitin code degrades deubiquitylation-protected substrates

Authors - Mai Morita
View Article
humanWB

PLoS Biol

A novel TOX3-WDR5-ABCG2 signaling axis regulates the progression of colorectal cancer by accelerating stem-like traits and chemoresistance

Authors - Jiaojiao Hao
View Article

Am J Cancer Res

PMID: 28123844

Authors - Jiaojiao Hao
View Article
humanWB

J Exp Clin Cancer Res

Melatonin synergizes BRAF-targeting agent vemurafenib in melanoma treatment by inhibiting iNOS/hTERT signaling and cancer-stem cell traits.

Authors - Jiaojiao Hao
View Article
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