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Validation Data Gallery

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  • Western Blot (WB) analysis of rat testis tissue using GTF2A1 Polyclonal antibody (21381-1-AP)

    WB analysis of rat testis using 21381-1-AP (same clone as 21381-1-PBS)

    rat testis tissue were subjected to SDS PAGE followed by western blot with 21381-1-AP (GTF2A1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21381-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of BGC-823 cells using GTF2A1 Polyclonal antibody (21381-1-AP)

    WB analysis of BGC-823 using 21381-1-AP (same clone as 21381-1-PBS)

    BGC-823 cells were subjected to SDS PAGE followed by western blot with 21381-1-AP (GTF2A1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21381-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SKOV-3 cells using GTF2A1 Polyclonal antibody (21381-1-AP)

    WB analysis of SKOV-3 using 21381-1-AP (same clone as 21381-1-PBS)

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 21381-1-AP (GTF2A1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21381-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using GTF2A1 Polyclonal antibody (21381-1-AP)

    IHC staining of human testis using 21381-1-AP (same clone as 21381-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 21381-1-AP (GTF2A1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 21381-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using GTF2A1 Polyclonal antibody (21381-1-AP)

    IHC staining of human testis using 21381-1-AP (same clone as 21381-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 21381-1-AP (GTF2A1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 21381-1-PBS in a different storage buffer formulation.

GTF2A1 Polyclonal antibody, PBS Only

GTF2A1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 21381-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21381-1-PBS targets GTF2A1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag15994

Product name: Recombinant human GTF2A1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-330 aa of BC107156

Sequence: MANSANTNTVPKLYRSVIEDVINDVRDIFLDDGVDEQVLMELKTLWENKLMQSRAVDGFHSEEQQLLLQVQQQHQPQQQQHHHHHHHQQAQPQQTVPQQAQTQQVLIPASQQATAPQVIVPDSKLIQHMNASNMSAAATAATLALPAGVTPVQQILTNSGQLLQVVRAANGAQYIFQPQQSVVLQQQVIPQMQPGGVQAPVIQQVLAPLPGGISPQTGVIIQPQQILFTGNKTQVIPTTVAAPTPAQAQITATGQQQPQAQPAQTQAPLVLQVDGTGDTSSEEDEDEEEDYDDDEEEDKEKDGAEDGQVEEEPLNSEDDVSDEEGQELFD

相同性解析による交差性が予測される生物種
Full Name general transcription factor IIA, 1, 19/37kDa
Calculated molecular weight 276 aa, 42 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC107156
Gene Symbol GTF2A1
Gene ID (NCBI) 2957
RRIDAB_2878853
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP52655
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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