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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GSTZ1 Polyclonal antibody (14889-1-AP)

    WB analysis using 14889-1-AP (same clone as 14889-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14889-1-AP (GSTZ1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14889-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using GSTZ1 Polyclonal antibody (14889-1-AP)

    WB analysis of mouse liver using 14889-1-AP (same clone as 14889-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 14889-1-AP (GSTZ1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14889-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using GSTZ1 Polyclonal antibody (14889-1-AP)

    IHC staining of mouse liver using 14889-1-AP (same clone as 14889-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14889-1-AP (GSTZ1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14889-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using GSTZ1 Polyclonal antibody (14889-1-AP)

    IHC staining of mouse liver using 14889-1-AP (same clone as 14889-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14889-1-AP (GSTZ1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14889-1-PBS in a different storage buffer formulation.

GSTZ1 Polyclonal antibody, PBS Only

GSTZ1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 14889-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14889-1-PBS targets GSTZ1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6676

Product name: Recombinant human GSTZ1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-216 aa of BC001453

Sequence: MQAGKPILYSYFRSSCSWRVRIALALKGIDYETVPINLIKDGGQQFSKDFQALNPMKQVPTLKIDGITIHQSLAIIEYLEEMRPTPRLLPQDPKKRASVRMISDLIAGGIQPLQNLSVLKQVGEEMQLTWAQNAITCGFNALEQILQSTAGIYCVGDEVTMADLCLVPQVANAERFKVDLTPYPTISSINKRLLVLEAFQVSHPCRQPDTPTELRA

相同性解析による交差性が予測される生物種
Full Name glutathione transferase zeta 1
Calculated molecular weight 24 kDa
Observed molecular weight 25-30 kDa
GenBank accession numberBC001453
Gene Symbol GSTZ1
Gene ID (NCBI) 2954
RRIDAB_2116363
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO43708
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Glutathione transferase zeta 1(GSTZ1) catalyzes the biotransformation of a range of α-haloacids, including dichloroacetic acid (DCA), and the penultimate step in the tyrosine degradation pathway. GSTZ1 is preferentially expressed in hepatocytes and renal proximal tubule cells where phenylalanine and tyrosine are catabolized(PMID: 16550164). This protein has 3 isoforms produced by alternative splicing.

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