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Validation Data Gallery

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  • Western Blot (WB) analysis of rat liver tissue using GSTT2 Polyclonal antibody (15063-1-AP)

    WB analysis of rat liver using 15063-1-AP (same clone as 15063-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 15063-1-AP (GSTT2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15063-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using GSTT2 Polyclonal antibody (15063-1-AP)

    WB analysis of mouse liver using 15063-1-AP (same clone as 15063-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 15063-1-AP (GSTT2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15063-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using GSTT2 Polyclonal antibody (15063-1-AP)

    WB analysis of L02 using 15063-1-AP (same clone as 15063-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 15063-1-AP (GSTT2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15063-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse lung tissue using GSTT2 Polyclonal antibody (15063-1-AP)

    WB analysis of mouse lung using 15063-1-AP (same clone as 15063-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 15063-1-AP (GSTT2 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 15063-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using GSTT2 Polyclonal antibody (15063-1-AP)

    IHC staining of human prostate cancer using 15063-1-AP (same clone as 15063-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15063-1-AP (GSTT2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15063-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using GSTT2 Polyclonal antibody (15063-1-AP)

    IHC staining of human prostate cancer using 15063-1-AP (same clone as 15063-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15063-1-AP (GSTT2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15063-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of L02 cells using GSTT2 Polyclonal antibody (15063-1-AP)

    IF Staining of L02 using 15063-1-AP (same clone as 15063-1-PBS)

    Immunofluorescent analysis of L02 cells using 15063-1-AP (GSTT2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15063-1-PBS in a different storage buffer formulation.

GSTT2 Polyclonal antibody, PBS Only

GSTT2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 15063-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15063-1-PBS targets GSTT2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7066

Product name: Recombinant human GSTT2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-244 aa of BC002415

Sequence: MGLELFLDLVSQPSRAVYIFAKKNGIPLELRTVDLVKGQHKSKEFLQINSLGKLPTLKDGDFILTESSAILIYLSCKYQTPDHWYPSDLQARARVHEYLGWHADCIRGTFGIPLWVQVLGPLIGVQVPEEKVERNRTAMDQALQWLEDKFLGDRPFLAGQQVTLADLMALEELMQPVALGYELFEGRPRLAAWRGRVEAFLGAELCQEAHSIILSILEQAAKKTLPTPSPEAYQAMLLRIARIP

相同性解析による交差性が予測される生物種
Full Name glutathione S-transferase theta 2
Calculated molecular weight 27 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC002415
Gene Symbol GSTT2
Gene ID (NCBI) 2953
RRIDAB_2878106
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP0CG29
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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