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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GSTT1 Monoclonal antibody (67645-1-Ig)

    WB analysis using 67645-1-Ig (same clone as 67645-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67645-1-Ig (GSTT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67645-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig liver tissue using GSTT1 Monoclonal antibody (67645-1-Ig)

    WB analysis of pig liver using 67645-1-Ig (same clone as 67645-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 67645-1-Ig (GSTT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67645-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using GSTT1 Monoclonal antibody (67645-1-Ig)

    WB analysis of K-562 using 67645-1-Ig (same clone as 67645-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 67645-1-Ig (GSTT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67645-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using GSTT1 Monoclonal antibody (67645-1-Ig)

    IHC staining of mouse liver using 67645-1-Ig (same clone as 67645-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67645-1-Ig (GSTT1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67645-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using GSTT1 Monoclonal antibody (67645-1-Ig)

    IHC staining of mouse liver using 67645-1-Ig (same clone as 67645-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67645-1-Ig (GSTT1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67645-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using GSTT1 Monoclonal antibody (67645-1-Ig)

    IF Staining of mouse liver using 67645-1-Ig (same clone as 67645-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using GSTT1 antibody (67645-1-Ig, Clone: 1A4C11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67645-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using GSTT1 Monoclonal antibody (67645-1-Ig)

    IF Staining of mouse liver using 67645-1-Ig (same clone as 67645-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using GSTT1 antibody (67645-1-Ig, Clone: 1A4C11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67645-1-PBS in a different storage buffer formulation.

GSTT1 Monoclonal antibody, PBS Only

GSTT1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 67645-1-PBS
Clone No.1A4C11

Host / Isotype

Mouse / IgG2b

Reactivity

Human, pig, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Glutathione transferase T1 1, GST class theta 1, GSTT1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67645-1-PBS targets GSTT1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, pig, mouse samples.

Tested Reactivity Human, pig, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag8573

Product name: Recombinant human GSTT1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-240 aa of BC007065

Sequence: MGLELYLDLLSQPCRAVYIFAKKNDIPFELRIVDLIKGQHLSDACAQVNPLKKVPALKDGDFTLTESVAILLYLTRKYKVPDYWYPQDLQARARVDEYLAWQHTTLRRSCLRALWHKVMFPVFLGEPVSPQTLAATLAELDVTLQLLEDKFLQNKAFLTGPHISLADLVAITELMHPVGAGCQVFEGRPKLATWRQRVEAAVGEDLFQEAHEVILKAKDFPPADPTIKQKLMPWVLAMIR

相同性解析による交差性が予測される生物種
Full Name glutathione S-transferase theta 1
Calculated molecular weight 240 aa, 27 kDa
Observed molecular weight 28 kDa, 30 kDa
GenBank accession numberBC007065
Gene Symbol GSTT1
Gene ID (NCBI) 2952
RRIDAB_2882845
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP30711
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

GSTT1 belongs to the GST superfamily and Theta family. It is a conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. GSTT1 acts on 1,2-epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4-nitrobenzyl chloride and 4-nitrophenethyl bromide. It displays glutathione peroxidase activity with cumene hydroperoxide. GSTM1 and GSTT1 has been suggested as a risk factor in various cancers. GSTT1 is important in the detoxification of unidentified xenobiotics in the large intestine.