Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of mouse liver using 16766-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16766-1-AP (GSTM4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16766-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16766-1-AP (GSTM4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 16766-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 16766-1-AP (GSTM4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse lung using 16766-1-AP
IP result of anti-GSTM4 (IP:16766-1-AP, 4ug; Detection:16766-1-AP 1:500) with mouse lung tissue lysate 4000 ug.
IHC staining of human colon cancer using 16766-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16766-1-AP (GSTM4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 16766-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16766-1-AP (GSTM4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 16766-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16766-1-AP (GSTM4 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 16766-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16766-1-AP (GSTM4 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 16766-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GSTM4 antibody (16766-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

GSTM4 Polyclonal antibody

GSTM4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 16766-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

GST class-mu 4, GST class mu 4, Glutathione S-transferase Mu 4, glutathione S transferase mu 4, EC:4.4.1.20

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse liver tissue, HeLa cells, mouse lung tissue
Positive IP detected in	mouse lung tissue
Positive IHC detected in	human colon cancer tissue, human cervical cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2400
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below
IHC	See 1 publications below

Product Information

16766-1-AP targets GSTM4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag10340 Product name: Recombinant human GSTM4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-218 aa of BC015513 Sequence: MSMTLGYWDIRGLAHAIRLLLEYTDSSYEEKKYTMGDAPDYDRSQWLNEKFKLGLDFPNLPYLIDGAHKITQSNAILCYIARKHNLCGETEEEKIRVDILENQAMDVSNQLARVCYSPDFEKLKPEYLEELPTMMQHFSQFLGKRPWFVGDKITFVDFLAYDVLDLHRIFEPNCLDAFPNLKDFISRFEGLEKISAYMKSSRFLPKPLYTRVAVWGNK 相同性解析による交差性が予測される生物種
Full Name	glutathione S-transferase mu 4
Calculated molecular weight	218 aa, 26 kDa
Observed molecular weight	26 kDa
GenBank accession number	BC015513
Gene Symbol	GSTM4
Gene ID (NCBI)	2948
RRID	AB_2116072
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q03013
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for GSTM4 antibody 16766-1-AP	Download protocol
IHC protocol for GSTM4 antibody 16766-1-AP	Download protocol
IP protocol for GSTM4 antibody 16766-1-AP	Download protocol
WB protocol for GSTM4 antibody 16766-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	J Gerontol A Biol Sci Med Sci PMID: 24285747 Authors - Rojanathammanee Lalida L View Article
mouse	WB	Age (Dordr) PMID: 25001375 Authors - Lalida Rojanathammanee View Article
human, mouse	WB	Mutat Res Black soybean seed coat polyphenols prevent B(a)P-induced DNA damage through modulating drug-metabolizing enzymes in HepG2 cells and ICR mice. Authors - Zhang Tianshun T View Article
human	WB,IHC	Reprod Sci 6-(7-Nitro-2,1,3-benzoxadiazol-4-ylthio) Hexanol Inhibits Proliferation and Induces Apoptosis of Endometriosis by Regulating Glutathione S-Transferase Mu Class 4 Authors - Wei Liu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse liver using 16766-1-AP

WB analysis of HeLa using 16766-1-AP

WB analysis of mouse lung using 16766-1-AP

IP experiment of mouse lung using 16766-1-AP

IHC staining of human colon cancer using 16766-1-AP

IHC staining of human colon cancer using 16766-1-AP

IHC staining of human cervical cancer using 16766-1-AP

IHC staining of human cervical cancer using 16766-1-AP

IF Staining of HepG2 using 16766-1-AP

GSTM4 Polyclonal antibody

GSTM4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

PMID: 24285747

PMID: 25001375

Black soybean seed coat polyphenols prevent B(a)P-induced DNA damage through modulating drug-metabolizing enzymes in HepG2 cells and ICR mice.

6-(7-Nitro-2,1,3-benzoxadiazol-4-ylthio) Hexanol Inhibits Proliferation and Induces Apoptosis of Endometriosis by Regulating Glutathione S-Transferase Mu Class 4