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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using GSTM3 Monoclonal antibody (67634-1-Ig)

    WB analysis of HepG2 using 67634-1-Ig (same clone as 67634-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67634-1-Ig (GSTM3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67634-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using GSTM3 Monoclonal antibody (67634-1-Ig)

    WB analysis of pig brain using 67634-1-Ig (same clone as 67634-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67634-1-Ig (GSTM3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67634-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using GSTM3 Monoclonal antibody (67634-1-Ig)

    WB analysis of HeLa using 67634-1-Ig (same clone as 67634-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67634-1-Ig (GSTM3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67634-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using GSTM3 Monoclonal antibody (67634-1-Ig)

    IF Staining of A431 using 67634-1-Ig (same clone as 67634-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using GSTM3 antibody (67634-1-Ig, Clone: 1E2B8 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67634-1-PBS in a different storage buffer formulation.

GSTM3 Monoclonal antibody, PBS Only

GSTM3 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67634-1-PBS
Clone No.1E2B8

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IF/ICC, ELISA

Synonyms

1E2B8, EC:2.5.1.18, Glutathione S transferase Mu 3, Glutathione S-transferase Mu 3, GST class mu 3

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67634-1-PBS targets GSTM3 in WB, IF/ICC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag7494

Product name: Recombinant human GSTM3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-225 aa of BC000088

Sequence: MSCESSMVLGYWDIRGLAHAIRLLLEFTDTSYEEKRYTCGEAPDYDRSQWLDVKFKLDLDFPNLPYLLDGKNKITQSNAILRYIARKHNMCGETEEEKIRVDIIENQVMDFRTQLIRLCYSSDHEKLKPQYLEELPGQLKQFSMFLGKFSWFAGEKLTFVDFLTYDILDQNRIFDPKCLDEFPNLKAFMCRFEALEKIAAYLQSDQFCKMPINNKMAQWGNKPVC

相同性解析による交差性が予測される生物種
Full Name glutathione S-transferase mu 3 (brain)
Calculated molecular weight 27 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC000088
Gene Symbol GSTM3
Gene ID (NCBI) 2947
RRIDAB_2882835
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP21266
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

GSTM3 is a cytosolic enzyme involved in prostaglandin and leukotriene synthesis and in the metabolization of toxic compounds, such as chemotherapeutic drugs, insecticides, herbicides, carcinogens, and by-products of oxidative stress. GSTM3 is expressed in testis, brain, lung, and lymphocytes. GSTM3 have been reported to be dysregulated in cancers like prostate cancer and colon cancer.

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