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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GSTM2 Polyclonal antibody

GSTM2 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 55474-1-AP

Synonyms

Glutathione S transferase Mu 2, GST class mu 2, GST4, GSTM, GSTM2, GSTM2 2, GTHMUS

Validation Data Gallery

Applications:
  • WB analysis using 55474-1-AP

    WB analysis using 55474-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55474-1-AP (GSTM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 55474-1-AP

    WB analysis using 55474-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55474-1-AP (GSTM2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 55474-1-AP

    IF Staining of HeLa using 55474-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GSTM2 antibody (55474-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inMCF-7 cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

55474-1-AP targets GSTM2 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name glutathione S-transferase mu 2 (muscle)
Calculated molecular weight 26 kDa
Observed molecular weight26 kDa
GenBank accession numberNM_000848
Gene symbol GSTM2
Gene ID (NCBI) 2946
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glutathione S-Transferase Mu 2 (GSTM2) is a phase II metabolizing enzyme that conjugates glutathione to electrophilic compounds. GSTM2 is closely related to cancer progression, and there have been several publications regarding the function of GSTM2 in drug resistance (PMID: 36038661). Western blot analysis detected GSTM2 at an apparent molecular mass of 26 kDa.

Protocols

Product Specific Protocols
WB protocol for GSTM2 antibody 55474-1-APDownload protocol
IF protocol for GSTM2 antibody 55474-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols