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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GSTK1 Recombinant monoclonal antibody (81527-1-RR)

    WB analysis using 81527-1-RR (same clone as 81527-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81527-1-RR (GSTK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81527-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using GSTK1 Recombinant monoclonal antibody (81527-1-RR)

    WB analysis of HepG2 using 81527-1-RR (same clone as 81527-1-PBS)

    WB result of GSTK1 antibody (81527-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSTK1 transfected HepG2 cells. This data was developed using the same antibody clone with 81527-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using GSTK1 Recombinant monoclonal antibody (81527-1-RR)

    IHC staining of mouse testis using 81527-1-RR (same clone as 81527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 81527-1-RR (GSTK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81527-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GSTK1 Recombinant monoclonal antibody (81527-1-RR)

    IHC staining of human liver cancer using 81527-1-RR (same clone as 81527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81527-1-RR (GSTK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81527-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using GSTK1 Recombinant monoclonal antibody (81527-1-RR)

    IHC staining of mouse liver using 81527-1-RR (same clone as 81527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 81527-1-RR (GSTK1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81527-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using GSTK1 Recombinant monoclonal antibody (81527-1-RR)

    IHC staining of human pancreas cancer using 81527-1-RR (same clone as 81527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 81527-1-RR (GSTK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81527-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using GSTK1 Recombinant monoclonal antibody (81527-1-RR)

    IHC staining of human lung cancer using 81527-1-RR (same clone as 81527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81527-1-RR (GSTK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81527-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using GSTK1 Recombinant monoclonal antibody (81527-1-RR)

    IHC staining of human stomach cancer using 81527-1-RR (same clone as 81527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 81527-1-RR (GSTK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81527-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using GSTK1 Recombinant monoclonal antibody (81527-1-RR)

    IF Staining of mouse liver using 81527-1-RR (same clone as 81527-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using GSTK1 antibody (81527-1-RR, Clone: 3J1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81527-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GSTK1 Recombinant monoclonal antibody (81527-1-RR)

    IF Staining of HeLa using 81527-1-RR (same clone as 81527-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GSTK1 antibody (81527-1-RR, Clone: 3J1 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81527-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

GSTK1 Recombinant monoclonal antibody, PBS Only

GSTK1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 81527-1-PBS
Clone No.3J1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

DsbA-L, EC:2.5.1.18, Glutathione S-transferase kappa 1, Glutathione S-transferase subunit 13, GST

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81527-1-PBS targets GSTK1 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag6036

Product name: Recombinant human GSTK1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-226 aa of BC050715

Sequence: MGPLPRTVELFYDVLSPYSWLGFEILCRYQNIWNINLQLRPSLITGIMKDSGNKPPGLLPRKGLYMANDLKLLRHHLQIPIHFPKDFLSVMLEKGSLSAMRFLTAVNLEHPEMLEKASRELWMRVWSRNEDITEPQSILAAAEKAGMSAEQAQGLLEKIATPKVKNQLKETTEAACRYGAFGLPITVAHVDGQTHMLFGSDRMELLAHLLGEKWMGPIPPAVNARL

相同性解析による交差性が予測される生物種
Full Name glutathione S-transferase kappa 1
Calculated molecular weight 32 kDa
Observed molecular weight27 kDa
GenBank accession numberBC050715
Gene Symbol GSTK1
Gene ID (NCBI) 373156
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9Y2Q3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

GSTK1, glutathione S-transferase kappa 1, belongs to the superfamily of glutathione S-transferase (GST), which is related to oxidative stress (PMID:16081649). GSTK1 expression is negatively correlated with obesity and hypertrophic cardiomyopathy (PMID:21428694, PMID:27378925). GSTK1 was expressed in the peroxisomal and mitochondrial fractions and localized to peroxisomes (PubMed:14742434).

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