Use Able AI chat for product recommendations

GST Tag Monoclonal antibody

GST Tag Monoclonal Antibody for WB, IP, ELISA
Cat No. 66001-2-Ig
Clone No.3G12B10

Host / Isotype

Mouse / IgG2a

Reactivity

Recombinant protein, Schistosoma japonicum and More (1)

Applications

WB, IF, IP, CoIP, ChIP, ELISA

GST, GST Tag

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
HRP
Size/Concentration: 

-/ -


ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。


国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inRecombinant protein
Positive IP detected inE. coli lysate

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66001-2-Ig targets GST Tag in WB, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with Recombinant protein, Schistosoma japonicum samples.

Tested Reactivity Recombinant protein, Schistosoma japonicum
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

Recombinant protein

相同性解析による交差性が予測される生物種
Full Name GST Tag
Calculated molecular weight 26 kDa
GenBank accession numberFN315687
Gene Symbol
Gene ID (NCBI)
RRIDAB_2881488
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. GST (Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody recognizes the GST-tag fused to either the N- or C-terminus of targeted proteins.

Protocols

Product Specific Protocols
IP protocol for GST Tag antibody 66001-2-IgDownload protocol
WB protocol for GST Tag antibody 66001-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Cell Metab

Aerobic glycolysis promotes tumor immune evasion by hexokinase2-mediated phosphorylation of IκBα

Authors - Dong Guo
WB,IP

Cell Metab

Hepatic FXR-FGF4 is required for bile acid homeostasis via an FGFR4-LRH-1 signal node under cholestatic stress

Authors - Lintao Song
WB

Cell Metab

Foxp3 confers long-term efficacy of chimeric antigen receptor-T cells via metabolic reprogramming

Authors - Congyi Niu
WB,IP

Mol Plant

The histone methyltransferase SUVR2 promotes DSB repair via chromatin remodeling and liquid-liquid phase separation.

Authors - Qianwen Liu
WB,IP

Cell Res

Targeting PTPN13 with 11-amino-acid peptides of C-terminal APC prevents immune evasion of colorectal cancer.

Authors - Wen-Hui Ma

Immunity

Lactate binds and inhibits the innate immune sensor STING to promote tumor immune evasion.

Authors - Dong Guo
Loading...
||
New chat

Able™

正在加载,请稍候...