Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, Jurkat cells, HeLa cells, mouse testis tissue |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84480-2-RR targets GSR in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag13080 Product name: Recombinant human GSR protein Source: e coli.-derived, PKG Tag: GST Domain: 274-522 aa of BC069244 Sequence: ISTNCTEELENAGVEVLKFSQVKEVKKTLSGLEVSMVTAVPGRLPVMTMIPDVDCLLWAIGRVPNTKDLSLNKLGIQTDDKGHIIVDEFQNTNVKGIYAVGDVCGKALLTPVAIAAGRKLAHRLFEYKEDSKLDYNNIPTVVFSHPPIGTVGLTEDEAIHKYGIENVKTYSTSFTPMYHAVTKRKTKCVMKMVCANKEEKVVGIHMQGLGCDEMLQGFAVAVKMGATKADFDNTVAIHPTSSEELVTLR 相同性解析による交差性が予測される生物種 |
| Full Name | glutathione reductase |
| Calculated molecular weight | 522 aa, 56 kDa |
| Observed molecular weight | 52-55 kDa |
| GenBank accession number | BC069244 |
| Gene Symbol | GSR |
| Gene ID (NCBI) | 2936 |
| RRID | AB_3672000 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P00390 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GSR(Glutathione reductase) is also named as GLUR, GRD1 and belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.It was first found in a black American a variant red cell GSR characterized by greater electrophoretic mobility and enzyme activity per unit of hemoglobin than the normal.This protein maintains high levels of reduced glutathione in the cytosol.It has 5 isoforms produced by alternative splicing and alternative initiation.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GSR antibody 84480-2-RR | Download protocol |
| WB protocol for GSR antibody 84480-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
