Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis of HepG2 using 16379-1-AP
WB result of GSNOR,ADH5 antibody (16379-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSNOR,ADH5 transfected HepG2 cells.
WB analysis using 16379-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 16379-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 16379-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 16379-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 16379-1-AP
IP result of anti-GSNOR,ADH5 (IP:16379-1-AP, 4ug; Detection:16379-1-AP 1:5000) with mouse liver tissue lysate 2400 ug.
IHC staining of human testis using 16379-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 16379-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human pancreas cancer using 16379-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human pancreas cancer using 16379-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human endometrial cancer using 16379-1-AP
Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:50 (under 25x lens).
IF Staining of HepG2 using 16379-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GSNOR,ADH5 antibody (16379-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
FC experiment of HepG2 using 16379-1-AP
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug GSNOR,ADH5 Polyclonal antibody (16379-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

GSNOR,ADH5 Polyclonal antibody

GSNOR,ADH5 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Cat No. 16379-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Synonyms

ADH5, ADH 3, ADH 5, ADH3, ADHX

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HepG2 cells, human brain tissue, human liver tissue, human testis tissue, mouse liver tissue, rat liver tissue
Positive IP detected in	mouse liver tissue
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 1 publications below

Product Information

16379-1-AP targets GSNOR,ADH5 in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9360 Product name: Recombinant human GSNOR,ADH5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-374 aa of BC014665 Sequence: MANEVIKCKAAVAWEAGKPLSIEEIEVAPPKAHEVRIKIIATAVCHTDAYTLSGADPEGCFPVILGHEGAGIVESVGEGVTKLKAGDTVIPLYIPQCGECKFCLNPKTNLCQKIRVTQGKGLMPDGTSRFTCKGKTILHYMGTSTFSEYTVVADISVAKIDPLAPLDKVCLLGCGISTGYGAAVNTAKLEPGSVCAVFGLGGVGLAVIMGCKVAGASRIIGVDINKDKFARAKEFGATECINPQDFSKPIQEVLIEMTDGGVDYSFECIGNVKVMRAALEACHKGWGVSVVVGVAASGEEIATRPFQLVTGRTWKGTAFGGWKSVESVPKLVSEYMSKKIKVDEFVTHNLSFDEINKAFELMHSGKSIRTVVKI 相同性解析による交差性が予測される生物種
Full Name	alcohol dehydrogenase 5 (class III), chi polypeptide
Calculated molecular weight	374 aa, 40 kDa
Observed molecular weight	40 kDa
GenBank accession number	BC014665
Gene Symbol	ADH5
Gene ID (NCBI)	128
RRID	AB_2289298
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P11766
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ADH5, also named as ADHX, FDH FALDH GSH-FDH ADH3 and GSNOR, belongs to the zinc-containing alcohol dehydrogenase family and Class-III subfamily. It is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. ADH5 mediates multiple cardiovascular functions. It plays in regulating heterocellular communication in the artery wall. (PMID:21071693). ADH5 immunostaining is distributed in both the nucleus and cytoplasm of the retinal ganglion cells(PMID:22117533).

Protocols

Product Specific Protocols
FC protocol for GSNOR,ADH5 antibody 16379-1-AP	Download protocol
IF protocol for GSNOR,ADH5 antibody 16379-1-AP	Download protocol
IHC protocol for GSNOR,ADH5 antibody 16379-1-AP	Download protocol
IP protocol for GSNOR,ADH5 antibody 16379-1-AP	Download protocol
WB protocol for GSNOR,ADH5 antibody 16379-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Int J Mol Sci Gene Copy Number and Post-Transductional Mechanisms Regulate TRAP1 Expression in Human Colorectal Carcinomas. Authors - Michele Pietrafesa KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HepG2 using 16379-1-AP

WB analysis using 16379-1-AP

WB analysis of human testis using 16379-1-AP

WB analysis of human liver using 16379-1-AP

WB analysis of human brain using 16379-1-AP

IP experiment of mouse liver using 16379-1-AP

IHC staining of human testis using 16379-1-AP