Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 11644-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11644-2-AP (Gelsolin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of U2OS using 11644-2-AP
U2OS (WT and GSN KO) lysates prepared with RIPA buffer, 25 μg protein loaded. 11644-2-AP incubated at 1:5000 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.
IP experiment of U2OS using 11644-2-AP
U2OS lysates prepared and IP of GSN performed using 1.0 μg of 11644-2-AP coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.
IHC staining of human colon cancer using 11644-2-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11644-2-AP (Gelsolin Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human colon cancer using 11644-2-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11644-2-AP (Gelsolin Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human colon using 11644-2-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11644-2-AP (Gelsolin antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 11644-2-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11644-2-AP (GSN antibody) at dilution of 1:50 (under 10x lens).

Featured Product
KD/KO Validated

Gelsolin Polyclonal antibody

Gelsolin Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 11644-2-AP

Publications(15)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

GSN, Actin-depolymerizing factor

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (15)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, U2OS cells, HeLa cells
Positive IP detected in	U2OS cells
Positive IHC detected in	human colon cancer tissue, human colon tissue, human prostate cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 9 publications below
IHC	See 4 publications below
IF	See 4 publications below

Product Information

11644-2-AP targets Gelsolin in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2239 Product name: Recombinant human Gelsolin protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 433-782 aa of BC026033 Sequence: MAAQHGMDDDGTGQKQIWRIEGSNKVPVDPATYGQFYGGDSYIILYNYRHGGRQGQIIYNWQGAQSTQDEVAASAILTAQLDEELGGTPVQSRVVQGKEPAHLMSLFGGKPMIIYKGGTSREGGQTAPASTRLFQVRANSAGATRAVEVLPKAGALNSNDAFVLKTPSAAYLWVGTGASEAEKTGAQELLRVLRAQPVQVAEGSEPDGFWEALGGKAAYRTSPRLKDKKMDAHPPRLFACSNKIGRFVIEEVPGELMQEDLATDDVMLLDTWDQVFVWVGKDSQEEEKTEALTSAKRYIETDPANRDRRTPITVVKQGFEPPSFVGWFLGWDDDYWSVDPLDRAMAELAA 相同性解析による交差性が予測される生物種
Full Name	gelsolin (amyloidosis, Finnish type)
Calculated molecular weight	782 aa, 86 kDa
Observed molecular weight	87-93 kDa
GenBank accession number	BC026033
Gene Symbol	Gelsolin
Gene ID (NCBI)	2934
RRID	AB_2295090
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P06396
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Gelsolin (GSN) is an actin binding protein that regulates actin-severing/depolymerizing in a calcium dependent manner, and acts as a multifunctional regulator for cell metabolism and survival. Gelsolin exists in two isoforms: a cytoplasmic gelsolin (cGSN; 80-90 kDa) and a plasma gelsolin (pGSN; 93 kDa), differing at N terminal residues. Decreased levels of pGSN are strongly associated with human diseases, such as, acute liver injury, major trauma, myocardial infarction, inflammation, lung injury, rheumatoid arthritis, hemodialysis, multiple sclerosis, Alzheimer's disease. Plasma gelsolin can interact with actin and form a complex of 130 kDa (PMID: 6330060, 3030756).

Protocols

Product Specific Protocols
IHC protocol for Gelsolin antibody 11644-2-AP	Download protocol
WB protocol for Gelsolin antibody 11644-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
		Cell Peripheral cancer attenuates amyloid pathology in Alzheimer's disease via cystatin-c activation of TREM2. Authors - Xinyan Li View Article
	IF	Mol Cell Noncanonical Modulation of the eIF2 Pathway Controls an Increase in Local Translation during Neural Wiring. Authors - Roberta Cagnetta View Article
human	IF	J Clin Invest Loss of RPGR disrupts motile cilia and causes primary ciliary dyskinesia by affecting F-actin dynamics. Authors - Yang Wu View Article
mouse	IHC	Sci Adv Cytoskeletal remodeling promotes tunneling nanotube formation and drives cardiac resident cell mitochondrial transfer in sepsis. Authors - Rui Song View Article
human	WB	Adv Healthc Mater Modification of PLGA Scaffold by MSC-Derived Extracellular Matrix Combats Macrophage Inflammation to Initiate Bone Regeneration via TGF-β-Induced Protein. Authors - Moyuan Deng View Article
human	IHC	Cell Rep Greater epithelial ridge cells are the principal organoid-forming progenitors of the mouse cochlea. Authors - Marie Kubota View Article

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Validation Data Gallery

WB analysis using 11644-2-AP

WB analysis of U2OS using 11644-2-AP

IP experiment of U2OS using 11644-2-AP

IHC staining of human colon cancer using 11644-2-AP

IHC staining of human colon cancer using 11644-2-AP

IHC staining of human colon using 11644-2-AP

IHC staining of human prostate cancer using 11644-2-AP

Gelsolin Polyclonal antibody

Gelsolin Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Peripheral cancer attenuates amyloid pathology in Alzheimer's disease via cystatin-c activation of TREM2.

Noncanonical Modulation of the eIF2 Pathway Controls an Increase in Local Translation during Neural Wiring.

Loss of RPGR disrupts motile cilia and causes primary ciliary dyskinesia by affecting F-actin dynamics.

Cytoskeletal remodeling promotes tunneling nanotube formation and drives cardiac resident cell mitochondrial transfer in sepsis.

Modification of PLGA Scaffold by MSC-Derived Extracellular Matrix Combats Macrophage Inflammation to Initiate Bone Regeneration via TGF-β-Induced Protein.

Greater epithelial ridge cells are the principal organoid-forming progenitors of the mouse cochlea.

Able^™

Validation Data Gallery

WB analysis using 11644-2-AP

WB analysis of U2OS using 11644-2-AP

IP experiment of U2OS using 11644-2-AP

IHC staining of human colon cancer using 11644-2-AP

IHC staining of human colon cancer using 11644-2-AP

IHC staining of human colon using 11644-2-AP

IHC staining of human prostate cancer using 11644-2-AP

Gelsolin Polyclonal antibody

Gelsolin Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Peripheral cancer attenuates amyloid pathology in Alzheimer's disease via cystatin-c activation of TREM2.

Noncanonical Modulation of the eIF2 Pathway Controls an Increase in Local Translation during Neural Wiring.

Loss of RPGR disrupts motile cilia and causes primary ciliary dyskinesia by affecting F-actin dynamics.

Cytoskeletal remodeling promotes tunneling nanotube formation and drives cardiac resident cell mitochondrial transfer in sepsis.

Modification of PLGA Scaffold by MSC-Derived Extracellular Matrix Combats Macrophage Inflammation to Initiate Bone Regeneration via TGF-β-Induced Protein.

Greater epithelial ridge cells are the principal organoid-forming progenitors of the mouse cochlea.

Able™

Able^™