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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GSG1L Polyclonal antibody

GSG1L Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17328-1-AP

Synonyms

GSG1 like, GSG1 like protein, GSG1L, PRO19651

Validation Data Gallery

Applications:
  • WB analysis of U-251 using 17328-1-AP

    WB analysis of U-251 using 17328-1-AP

    U-251 cells were subjected to SDS PAGE followed by western blot with 17328-1-AP (GSG1L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis using 17328-1-AP

    WB analysis using 17328-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17328-1-AP (GSG1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 17328-1-AP

    IHC staining of human tonsillitis using 17328-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17328-1-AP (GSG1L Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human tonsillitis using 17328-1-AP

    IHC staining of human tonsillitis using 17328-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17328-1-AP (GSG1L Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inRaji cells, U-251 cells, SGC-7901 cells, mouse brain tissue, mouse liver tissue, rat brain tissue, rat liver tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

17328-1-AP targets GSG1L in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GSG1L fusion protein Ag11307 相同性解析による交差性が予測される生物種
Full Name GSG1-like
Calculated molecular weight 176 aa, 21 kDa
Observed molecular weight 36-43 kDa
GenBank accession numberBC016460
Gene symbol GSG1L
Gene ID (NCBI) 146395
RRIDAB_2878384
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GSG1L is a component of the inner core of AMPAR complex. It modifies AMPA receptor (AMPAR) gating. GSG1L is mainly present on B lymphocytes. This antibody can recognize all the 4 isoforms of GSG1L. GSG1L was detected the isoform of 43 kDa in rat brain (PMID: 22813734).

Protocols

Product Specific Protocols
WB protocol for GSG1L antibody 17328-1-APDownload protocol
IHC protocol for GSG1L antibody 17328-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
rat

Nat Commun

GSG1L-containing AMPA receptor complexes are defined by their spatiotemporal expression, native interactome and allosteric sites

Authors - Amanda M Perozzo
View Article