Validation Data Gallery
Tested Applications
| Positive WB detected in | Raji cells, U-251 cells, SGC-7901 cells, mouse brain tissue, mouse liver tissue, rat brain tissue, rat liver tissue |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17328-1-AP targets GSG1L in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11307 Product name: Recombinant human GSG1L protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-176 aa of BC016460 Sequence: MCLELFHSSNVIDGLKLNAFAAVFTVLSGLLGMVAHMMYTQVFQVTVSLGPEDWRPHSWDYGWSFCLAWGSFTCCMAASVTTLNSYTKTVIEFRHKRKVFEQGYREEPTFIDPEAIKYFRERMEKRDGSEEDFHLDCRHERYPARHQPHMADSWPRSSAQEAPELNRQCWVLGHWV 相同性解析による交差性が予測される生物種 |
| Full Name | GSG1-like |
| Calculated molecular weight | 176 aa, 21 kDa |
| Observed molecular weight | 36-43 kDa |
| GenBank accession number | BC016460 |
| Gene Symbol | GSG1L |
| Gene ID (NCBI) | 146395 |
| RRID | AB_2878384 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6UXU4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GSG1L is a component of the inner core of AMPAR complex. It modifies AMPA receptor (AMPAR) gating. GSG1L is mainly present on B lymphocytes. This antibody can recognize all the 4 isoforms of GSG1L. GSG1L was detected the isoform of 43 kDa in rat brain (PMID: 22813734).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GSG1L antibody 17328-1-AP | Download protocol |
| WB protocol for GSG1L antibody 17328-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
