Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse testis tissue |
| Positive IF-P detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15258-1-AP targets GSG1 in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7200 Product name: Recombinant human GSG1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-282 aa of BC001796 Sequence: MELSKAFSGQRTLLSAILSMLSLSFSTTSLLSNYWFVGTQKVPKPLCEKGLAAKCFDMPVSLDGDTNTSTQEVVQYNWETGDDRFSFRSFRSGMWLSCEETVEEPGERCRSFIELTPPAKRGEKGLLEFATLQGPCHPTLRFGGKRLMEKASLPSPPLGLCGKNPMVIPGNADHLHRTSIHQLPPATNRLATHWEPCLWAQTERLCCCFLCPVRSPGDGGPHDVFTSLPSDCQLGSRRLETTCLELWLGLLHGLALLHLLHGVGCHHLQHVHQDGAGVQVQA 相同性解析による交差性が予測される生物種 |
| Full Name | germ cell associated 1 |
| Calculated molecular weight | 37 kDa |
| Observed molecular weight | 39 kDa |
| GenBank accession number | BC001796 |
| Gene Symbol | GSG1 |
| Gene ID (NCBI) | 83445 |
| RRID | AB_3085457 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q2KHT4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GSG1 causes the redistribution of PAPOLB from the cytosol to the endoplasmic reticulum. It has eight isoforms with MW 31-33 kDa and 36-40 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GSG1 antibody 15258-1-AP | Download protocol |
| WB protocol for GSG1 antibody 15258-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |