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Validation Data Gallery

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  • Western Blot (WB) analysis of THP-1 cells using GSDMD (C-terminal) Polyclonal antibody (30531-1-AP)

    WB analysis of THP-1 using 30531-1-AP

    THP-1 cells were subjected to SDS PAGE followed by western blot with 30531-1-AP (GSDMD (C-terminal) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using GSDMD Polyclonal antibody (30531-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 30531-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 30531-1-AP (GSDMD antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using GSDMD Polyclonal antibody (30531-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 30531-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 30531-1-AP (GSDMD antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using GSDMD Polyclonal antibody (30531-1-AP)

    IHC staining of mouse lung using 30531-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 30531-1-AP (GSDMD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using GSDMD Polyclonal antibody (30531-1-AP)

    IHC staining of mouse lung using 30531-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 30531-1-AP (GSDMD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GSDMD Polyclonal antibody

GSDMD Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30531-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

FKSG10, gasdermin D, Gasdermin domain-containing protein 1, gasderminD, Gasdermin-D

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inTHP-1 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30531-1-AP targets GSDMD in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag33627

Product name: Recombinant human GSDMD protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 276-484 aa of BC008904

Sequence: GVPAEGAFTEDFQGLRAEVETISKELELLDRELCQLLLEGLEGVLRDQLALRALEEALEQGQSLGPVEPLDGPAGAVLECLVLSSGMLVPELAIPVVYLLGALTMLSETQHKLLAEALESQTLLGPLELVGSLLEQSAPWQERSTMSLPPGLLGNSWGEGAPAWVLLDECGLELGEDTPHVCWEPQAQGRMCALYASLALLSGLSQEPH

相同性解析による交差性が予測される生物種
Full Name gasdermin D
Calculated molecular weight 484 aa, 53 kDa
Observed molecular weight53 kDa, 43 kDa
GenBank accession numberBC008904
Gene Symbol GSDMD
Gene ID (NCBI) 79792
RRIDAB_3086353
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP57764
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GSDMD is a 53 kDa cytosolic protein and a member of the gasdermin protein family, which features 6 members in humans (GSDMA, -B, -C, -D, -E and -F. GSDMD was identified as the executioner of inflammasome-associated pyroptosis. Caspase-1, -11, or -4 cleave GSDMD after an aspartate residue within a central linker domain (D275 in humans and D276 in mice) to generate a 31-kDa N-terminal GSDMD fragment (GSDMD-NT) and a 22-kDa C-terminal fragment (GSDMD-CT). In addition to the canonical 31/22 kDa cleavage products, a 43 kDa fragment has been observed under specific experimental or pathological conditions. The generation of the 43 kDa fragment was observed upon caspase-3 and -7 activation during apoptosis. This antibody can detect 53 kDa full-length GSDMD and 43 kDa fragment as described in the literature (PMID: 28392147, PMID: 28979266).

Protocols

Product Specific Protocols
IHC protocol for GSDMD antibody 30531-1-APDownload protocol
WB protocol for GSDMD antibody 30531-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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