Validation Data Gallery
Tested Applications
Positive WB detected in | A431 cells, NIH/3T3 cells, Jurkat cells, C6 cells, rat liver tissue, rat brain tissue |
Positive IP detected in | THP-1 cells |
Positive IHC detected in | human ovary cancer tissue, mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | Jurkat cells |
This antibody can only dectect full length GSDMD.
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 7 publications below |
WB | See 285 publications below |
IHC | See 74 publications below |
IF | See 98 publications below |
IP | See 1 publications below |
Product Information
20770-1-AP targets GSDMD in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, pig, rabbit, bovine, hamster |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | GSDMD fusion protein Ag14757 相同性解析による交差性が予測される生物種 |
Full Name | gasdermin D |
Calculated molecular weight | 484 aa, 53 kDa |
Observed molecular weight | 50-53 kDa |
GenBank accession number | BC008904 |
Gene Symbol | GSDMD |
Gene ID (NCBI) | 79792 |
RRID | AB_10696319 |
Conjugate | Unconjugated |
Form | |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P57764 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GSDMD is a 53 kDa cytosolic protein and a member of the gasdermin protein family, which features 6 members in humans (GSDMA, -B, -C, -D, -E and -F. GSDMD was identified as the executioner of inflammasome-associated pyroptosis. Caspase-1, -11, or -4 cleave GSDMD after an aspartate residue within a central linker domain (D275 in humans and D276 in mice) to generate a 31-kDa N-terminal GSDMD fragment (GSDMD-NT) and a 22-kDa C-terminal fragment (GSDMD-CT). In addition to the canonical 31/22 kDa cleavage products, a 43 kDa fragment has been observed under specific experimental or pathological conditions. The generation of the 43 kDa fragment was observed upon caspase-3 and -7 activation during apoptosis. This antibody can only dectect full length GSDMD.
Protocols
Product Specific Protocols | |
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WB protocol for GSDMD antibody 20770-1-AP | Download protocol |
IHC protocol for GSDMD antibody 20770-1-AP | Download protocol |
IF protocol for GSDMD antibody 20770-1-AP | Download protocol |
IP protocol for GSDMD antibody 20770-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Type III interferons induce pyroptosis in gut epithelial cells and impair mucosal repair | ||
Nature Inflammasome-activated gasdermin D causes pyroptosis by forming membrane pores.
| ||
ACS Nano Engineering Extracellular Vesicles Restore the Impaired Cellular Uptake and Attenuate Intervertebral Disc Degeneration. | ||
Acta Pharm Sin B Miltirone induces cell death in hepatocellular carcinoma cell through GSDME-dependent pyroptosis. | ||
Acta Pharm Sin B A high-throughput Gaussia luciferase reporter assay for screening potential gasdermin E activators against pancreatic cancer | ||
Cell Res Gasdermin D is an executor of pyroptosis and required for interleukin-1β secretion. |