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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using GSDMB Monoclonal antibody (67527-1-Ig)

    WB analysis of MCF-7 using 67527-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67527-1-Ig (GSDMB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using GSDMB Monoclonal antibody (67527-1-Ig)

    WB analysis of PC-3 using 67527-1-Ig

    PC-3 cells were subjected to SDS PAGE followed by western blot with 67527-1-Ig (GSDMB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using GSDMB Monoclonal antibody (67527-1-Ig)

    WB analysis of COLO 320 using 67527-1-Ig

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 67527-1-Ig (GSDMB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GSDMB Monoclonal antibody (67527-1-Ig)

    IF Staining of HepG2 using 67527-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GSDMB antibody (67527-1-Ig, Clone: 2F12C4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

GSDMB Monoclonal antibody

GSDMB Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67527-1-Ig
Clone No.2F12C4
Publications(2)

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IF/ICC, ELISA

Synonyms

gasdermin B, Gasdermin like protein, GSDMB, GSDML, PP4052, PRO2521

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, COLO 320 cells, PC-3 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67527-1-Ig targets GSDMB in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag28332

Product name: Recombinant human GSDMB protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-385 aa of BC025682

Sequence: MFSVFEEITRIVVKEMDAGGDMIAVRSLVDADRFRCFHLVGEKRTFFGCRHYTTGLTLMDILDTDGDKWLDELDSGLQGQKAEFQILDNVDSTGELIVRLPKEITISGSFQGFHHQKIKISENRISQQYLATLENRKLKRELPFSFRSINTRENLYLVTETLETVKEETLKSDRQYKFWSQISQGHLSYKHKGQREVTIPPNRVLSYRVKQLVFPNKETMSAGLDIHFRGKTKSFPEGKSLGSEDSRNMKEKLEDMESVLKDLTEEKRKDVLNSLAKCLGKEDIRQDLEQRVSEVLISRELHMEDSDKPLLSSLFNAAGVLVEARAKAILDFLDALLELSEEQQFVAEALEKGTLPLLKDQVKSVMEQNWDELASSPPDMDYDPE

相同性解析による交差性が予測される生物種
Full Name gasdermin B
Calculated molecular weight 411 aa, 47 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC025682
Gene Symbol GSDMB
Gene ID (NCBI) 55876
RRIDAB_2882746
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ8TAX9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for GSDMB antibody 67527-1-IgDownload protocol
WB protocol for GSDMB antibody 67527-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Biochem Pharmacol

Overexpression of GJB1-13k induces mitochondrial calcium overload and triggers apoptosis and GSDME-mediated pyroptosis in cervical cancer.

Authors - Shaoyi Cai
View Article
humanWB,IF

Arch Biochem Biophys

GSDMB involvement in the pathogenesis of abdominal aortic aneurysm through regulation of macrophage non-canonical pyroptosis

Authors - Xiaoping Xie
View Article
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