Validation Data Gallery
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Tested Applications
| Positive WB detected in | A431 cells |
| Positive IHC detected in | human liver cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IHC | See 4 publications below |
| IF | See 1 publications below |
Product Information
12885-1-AP targets GSDMB in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GSDMB fusion protein Ag3895 相同性解析による交差性が予測される生物種 |
| Full Name | gasdermin B |
| Calculated molecular weight | 411 aa, 47 kDa |
| Observed molecular weight | 47 kDa |
| GenBank accession number | BC025682 |
| Gene Symbol | GSDMB |
| Gene ID (NCBI) | 55876 |
| RRID | AB_2114762 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TAX9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GSDMB antibody 12885-1-AP | Download protocol |
| IHC protocol for GSDMB antibody 12885-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Am J Respir Cell Mol Biol Glutaminolysis Epigenetically Regulates Anti-Apoptotic Gene Expression in IPF Fibroblasts. | ||
Front Immunol Crosstalk of necroptosis and pyroptosis defines tumor microenvironment characterization and predicts prognosis in clear cell renal carcinoma
| ||
Int J Biol Sci USP24-GSDMB complex promotes bladder cancer proliferation via activation of the STAT3 pathway. | ||
Front Oncol Novel Pyroptosis-Related Gene Signatures Identified as the Prognostic Biomarkers for Bladder Carcinoma. | ||
J Biol Chem Glutaminolysis is required for transforming growth factor-β1-induced myofibroblast differentiation and activation. | ||
J Allergy Clin Immunol A functional splice variant associated with decreased asthma risk abolishes the ability of gasdermin B to induce epithelial cell pyroptosis. |