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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GSDMA Recombinant antibody

GSDMA Recombinant Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

230161D4

Cat no : 82920-1-RR

Synonyms

FKSG9, FLJ39120, Gasdermin 1, gasdermin A, GSDM, GSDM1, GSDMA

Validation Data Gallery

Applications:
  • WB analysis of Daudi using 82920-1-RR

    WB analysis of Daudi using 82920-1-RR

    Daudi cells were subjected to SDS PAGE followed by western blot with 82920-1-RR (GSDMA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 82920-1-RR

    IF Staining of HeLa using 82920-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GSDMA antibody (82920-1-RR, Clone: 230161D4 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Tested Applications

Positive WB detected inDaudi cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:125-1:500
Sample-dependent, check data in validation data gallery

Product Information

82920-1-RR targets GSDMA in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GSDMA fusion protein Ag24407 相同性解析による交差性が予測される生物種
Full Name gasdermin A
Calculated molecular weight 445 aa, 49 kDa
Observed molecular weight49 kDa
GenBank accession numberBC109197
Gene symbol GSDMA
Gene ID (NCBI) 284110
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Gasdermins, a family of five pore-forming proteins (GSDMA-GSDME) in humans expressed predominantly in the skin, mucosa and immune sentinel cells, are key executioners of inflammatory cell death (pyroptosis), which recruits immune cells to infection sites and promotes protective immunity. GSDMA was predicted to enable phosphatidylinositol-4,5-bisphosphate binding activity, phosphatidylinositol-4-phosphate binding activity, and phosphatidylserine binding activity. Involved in apoptotic process.

Protocols

Product Specific Protocols
WB protocol for GSDMA antibody 82920-1-RRDownload protocol
IF protocol for GSDMA antibody 82920-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols