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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BET1L Polyclonal antibody (14163-1-AP)

    WB analysis using 14163-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14163-1-AP (BET1L antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using BET1L Polyclonal antibody (14163-1-AP)

    IHC staining of human cervical cancer using 14163-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 14163-1-AP (BET1L antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using BET1L Polyclonal antibody (14163-1-AP)

    IHC staining of human cervical cancer using 14163-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14163-1-AP (BET1L antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using BET1L Polyclonal antibody (14163-1-AP)

    IHC staining of human cervical cancer using 14163-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14163-1-AP (BET1L antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using BET1L Polyclonal antibody (14163-1-AP)

    IHC staining of mouse stomach using 14163-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 14163-1-AP (BET1L antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using BET1L Polyclonal antibody (14163-1-AP)

    IHC staining of mouse stomach using 14163-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 14163-1-AP (BET1L antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

BET1L Polyclonal antibody

BET1L Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14163-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Vesicle transport protein GOS15, GS15, GOS-15, Golgi SNARE with a size of 15 kDa, BET1-like protein

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, K-562 cells, MCF-7 cells
Positive IHC detected inhuman cervical cancer tissue, mouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14163-1-AP targets BET1L in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5351

Product name: Recombinant human GS15 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-102 aa of BC032779

Sequence: MADWARAQSPGAVEEILDRENKRMADSLASKVTRLKSLALDIDRDAEDQNRYLDGMVRAHGVRVSVPCPSTTCCRACSVSFSTGAGGWSNHHFCVILLGFLP

相同性解析による交差性が予測される生物種
Full Name blocked early in transport 1 homolog (S. cerevisiae)-like
Calculated molecular weight 102 aa, 11 kDa
Observed molecular weight13-15 kDa
GenBank accession numberBC032779
Gene Symbol BET1L
Gene ID (NCBI) 51272
RRIDAB_2064737
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NYM9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for BET1L antibody 14163-1-APDownload protocol
WB protocol for BET1L antibody 14163-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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