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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using GRWD1 Polyclonal antibody (10354-1-AP)

    WB analysis of HEK-293 using 10354-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10354-1-AP (GRWD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using GRWD1 Polyclonal antibody (10354-1-AP)

    WB analysis of PC-3 using 10354-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 10354-1-AP (GRWD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using GRWD1 Polyclonal antibody (10354-1-AP)

    WB analysis of HEK-293 using 10354-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10354-1-AP (GRWD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using GRWD1 Polyclonal antibody (10354-1-AP)

    WB analysis of HEK-293 using 10354-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10354-1-AP (GRWD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using GRWD1 Polyclonal antibody (10354-1-AP)

    WB analysis of PC-3 using 10354-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 10354-1-AP (GRWD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using GRWD1 Polyclonal antibody (10354-1-AP)

    WB analysis of PC-3 using 10354-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 10354-1-AP (GRWD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using GRWD1 Polyclonal antibody (10354-1-AP)

    WB analysis of HEK-293 using 10354-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10354-1-AP (GRWD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using GRWD1 Polyclonal antibody (10354-1-AP)

    WB analysis of HEK-293 using 10354-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10354-1-AP (GRWD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SKOV-3 cells using GRWD1 Polyclonal antibody (10354-1-AP)

    WB analysis of SKOV-3 using 10354-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10354-1-AP (GRWD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of PC-3 cells using GRWD1 Polyclonal antibody (10354-1-AP)

    IP experiment of PC-3 using 10354-1-AP

    IP result of anti-GRWD1 (IP:10354-1-AP, 4ug; Detection:10354-1-AP 1:1000) with PC-3 cells lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using GRWD1 Polyclonal antibody (10354-1-AP)

    IHC staining of human prostate cancer using 10354-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10354-1-AP (GRWD1 antibody) at dilution of 1:100 (under 25x lens).

GRWD1 Polyclonal antibody

GRWD1 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 10354-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Synonyms

Glutamate-rich WD repeat-containing protein 1, GRWD, KIAA1942, RRB1, WDR28

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHEK-293 cells, PC-3 cells, SKOV-3 cells
Positive IP detected inPC-3 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10354-1-AP targets GRWD1 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0367

Product name: Recombinant human GRWD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 20-236 aa of BC002440

Sequence: AESGDTSSEGPAQVYLPGRGPPLREGEELVMDEEAYVLYHRAQTGAPCLSFDIVRDHLGDNRTELPLTLYLCAGTQAESAQSNRLMMLRMHNLHGTKPPPSEGSDEEEEEEDEEDEEERKPQLELAMVPHYGGINRVRVSWLGEEPVAGVWSEKGQVEVFALRRLLQVVEEPQALAAFLRDEQAQMKPIFSFAGHMGEGFALDWSPRVTGRLLTG

相同性解析による交差性が予測される生物種
Full Name glutamate-rich WD repeat containing 1
Calculated molecular weight 49 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC002440
Gene Symbol GRWD1
Gene ID (NCBI) 83743
RRIDAB_2114642
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BQ67
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

WD-repeat proteins are a class of functionally divergent molecules that cooperate with other proteins to regulate cellular processes. WD-repeat proteins have been implicated in a variety of cellular functions that are mostly regulatory in nature, including cytoskeleton organization, vesicular fusion, pre-mRNA splicing, and cell cycle regulation. WD-repeat-containing protein designated glutamate-rich WD repeat (GRWD1) is a component of the 50S and 80S preribosomal complexes and plays a role in ribosome biogenesis and during myeloid differentiation its levels are regulated. (PMID:15885502)

Protocols

Product Specific Protocols
WB protocol for GRWD1 antibody 10354-1-APDownload protocol
IHC protocol for GRWD1 antibody 10354-1-APDownload protocol
IP protocol for GRWD1 antibody 10354-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Exp Cell Res

GRWD1 promotes cell proliferation and migration in non-small cell lung cancer by activating the Notch pathway.

Authors - Qiongzi Wang
View Article