GRP94 Monoclonal antibody
GRP94 Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (2)
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
4G7C7
Cat no : 60012-2-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells |
| Positive IHC detected in | human colon cancer tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells, A431 cells |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 4 publications below |
Product Information
60012-2-Ig targets GRP94 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | GRP94 fusion protein Ag1439 相同性解析による交差性が予測される生物種 |
| Full Name | heat shock protein 90kDa beta (Grp94), member 1 |
| Calculated molecular weight | 96 kDa |
| Observed molecular weight | 95 kDa |
| GenBank accession number | BC009195 |
| Gene symbol | HSP90B1 |
| Gene ID (NCBI) | 7184 |
| RRID | AB_2881351 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HSP90 proteins are highly conserved molecular chaperones, which normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 (GP96 or GRP94) is an endoplasmic reticulum paralogue of the cytosolic HSP90. As a major ER chaperone to mediate the UPR and a master chaperone for Toll-like receptors (TLRs), HSP90b1 chaperones peptides to MHC class I molecules of dendritic cells and other antigen-presenting cells, as well as facilitating the assembly of immunoglobulin. The protein is also involved in many other bio-processes. This antibody was generated against the N-terminal region of full-length HSP90b1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GRP94 antibody 60012-2-Ig | Download protocol |
| IHC protocol for GRP94 antibody 60012-2-Ig | Download protocol |
| IF protocol for GRP94 antibody 60012-2-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Control Release Sertoli cell-derived extracellular vesicles traverse the blood-testis barrier and deliver miR-24-3p inhibitor into germ cells improving sperm mobility | ||
Anticancer Drugs Berberine inhibits proliferation and migration of colorectal cancer cells by downregulation of GRP78. | ||
J Transl Med Comprehensive profiling of extracellular vesicles in uveitis and scleritis enables biomarker discovery and mechanism exploration | ||
Virol Sin Hsp90 β is Critical for the Infection of Severe Fever with Thrombocytopenia Syndrome Virus |
