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GRP78/BIP Polyclonal antibody
GRP78/BIP Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 11587-1-AP
Validation Data Gallery
|Positive WB detected in||HEK-293 cells, BxPC-3 cells, Caco-2 cells, HeLa cells, HepG2 cells, HT-29 cells, mouse liver, rat liver|
|Positive IP detected in||MCF-7 cells|
|Positive IHC detected in||human pancreas cancer tissue, mouse kidney tissue, human breast cancer tissue, rat brain tissue, human colon cancer tissue, mouse heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11587-1-AP targets GRP78/BIP in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||bovine, carp, chicken, deer, fish, Hamster, human, medaka embryos, mouse, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GRP78/BIP fusion protein Ag2188|
|Full Name||heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)|
|Calculated molecular weight||78 kDa|
|Observed molecular weight||70-78 kDa|
|GenBank accession number||BC020235|
|Gene ID (NCBI)||3309|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).It is a constitutively expressed resident protein of the ER in all eukaryotic cells. Recently it has been reported that GRP78 is associated with apoptosis or inhibition of cancer cell growth. 11587-1-AP antibody recognizes one or two bands with MW 70-78 kDa for the signal peptide of GRP78 protein can be finally cleaved.
|Product Specific Protocols|
|WB protocol for GRP78/BIP antibody 11587-1-AP||Download protocol|
|IHC protocol for GRP78/BIP antibody 11587-1-AP||Download protocol|
|IF protocol for GRP78/BIP antibody 11587-1-AP||Download protocol|
|IP protocol for GRP78/BIP antibody 11587-1-AP||Download protocol|
|FC protocol for GRP78/BIP antibody 11587-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
PK (Verified Customer) (03-22-2022)
Yeawon (Verified Customer) (03-17-2022)
Works very good in mouse kidney tissue.
Emma (Verified Customer) (03-15-2022)
Works well by WB in LNCaP and CWR22Rv1 cells @ 1:1000. A single band observed.
P (Verified Customer) (05-28-2021)
Kevin (Verified Customer) (01-24-2019)
Works pretty good in HepG2 cells. One single band was observed.
Sylvain (Verified Customer) (12-03-2018)
Natalie (Verified Customer) (11-20-2018)