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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GRP75 Recombinant monoclonal antibody (86721-1-RR)

    WB analysis using 86721-1-RR (same clone as 86721-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86721-1-RR (GRP75 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86721-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GRP75 Recombinant monoclonal antibody (86721-1-RR)

    WB analysis using 86721-1-RR (same clone as 86721-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86721-1-RR (GRP75 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86721-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86721-1-RR

    Affinity and Kinetic Characterization of 86721-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86721-1-RR against Human GRP75 were performed. The affinity constant is 1.66 nM.

GRP75 Recombinant monoclonal antibody, PBS Only

GRP75 Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 86721-1-PBS
Clone No.251670D1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

HSPA9, 75 kDa glucose-regulated protein, CSA, GRP-75, Heat shock 70 kDa protein 9

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86721-1-PBS targets GRP75 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag6673

Product name: Recombinant human GRP75 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 331-679 aa of BC000478

Sequence: YLTMDSSGPKHLNMKLTRAQFEGIVTDLIRRTIAPCQKAMQDAEVSKSDIGEVILVGGMTRMPKVQQTVQDLFGRAPSKAVNPDEAVAIGAAIQGGVLAGDVTDVLLLDVTPLSLGIETLGGVFTKLINRNTTIPTKKSQVFSTAADGQTQVEIKVCQGEREMAGDNKLLGQFTLIGIPPAPRGVPQIEVTFDIDANGIVHVSAKDKGTGREQQIVIQSSGGLSKDDIENMVKNAEKYAEEDRRKKERVEAVNMAEGIIHDTETKMEEFKDQLPADECNKLKEEISKMRELLARKDSETGENIRQAASSLQQASLKLFEMAYKKMASEREGSGSSGTGEQKEDQKEEKQ

相同性解析による交差性が予測される生物種
Full Name heat shock 70kDa protein 9 (mortalin)
Calculated molecular weight 74 kDa
Observed molecular weight 70-75 kDa
GenBank accession numberBC000478
Gene Symbol GRP75
Gene ID (NCBI) 3313
RRIDAB_3745077
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP38646
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

GRP75 (also known as mortalin, HSPA9 or mt-Hsp70) is a constitutively expressed member of the HSPA (HSP70) family of heat-shock proteins. It is located in the mitochondrial matrix and anti-GRP75 is commonly used as the marker for mitochondria. It has been reported that GRP75 is enriched in cancer cells and contributes to carcinogenesis. In addition, decreased expression level of GRP75 has been found in neurodegenerative disorders like Parkinson's disease. (PMID: 21640711, 22920904)

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