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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

GRK6 Polyclonal antibody

GRK6 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11439-1-AP

Synonyms

GPRK6, GRK6

Validation Data Gallery

Applications:
  • WB analysis of HEK-293 using 11439-1-AP

    WB analysis of HEK-293 using 11439-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11439-1-AP (GRK6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of human kidney using 11439-1-AP

    WB analysis of human kidney using 11439-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 11439-1-AP (GRK6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 11439-1-AP

    IP experiment of HEK-293 using 11439-1-AP

    IP result of anti-GRK6 (IP:11439-1-AP, 4ug; Detection:11439-1-AP 1:500) with HEK-293 cells lysate 1120ug.

  • IHC staining of human renal cell carcinoma using 11439-1-AP

    IHC staining of human renal cell carcinoma using 11439-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 11439-1-AP (GRK6 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human renal cell carcinoma using 11439-1-AP

    IHC staining of human renal cell carcinoma using 11439-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 11439-1-AP (GRK6 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHEK-293 cells, human kidney tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11439-1-AP targets GRK6 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GRK6 fusion protein Ag1992 相同性解析による交差性が予測される生物種
Full Name G protein-coupled receptor kinase 6
Calculated molecular weight 66 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC017272
Gene symbol GRK6
Gene ID (NCBI) 2870
RRIDAB_2248070
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GRK6 antibody 11439-1-APDownload protocol
IHC protocol for GRK6 antibody 11439-1-APDownload protocol
IP protocol for GRK6 antibody 11439-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Phospho-selective mechanisms of arrestin conformations and functions revealed by unnatural amino acid incorporation and (19)F-NMR.

Authors - Fan Yang
  • KD Validated
View Article
humanWB,IHC

Front Oncol

GRK6 Depletion Induces HIF Activity in Lung Adenocarcinoma.

Authors - Sumei Yao
View Article