Validation Data Gallery
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Tested Applications
| Positive WB detected in | pig brain tissue, rabbit cerebellum tissue, rat brain tissue, mouse brain tissue, chicken brain tissue |
| Positive IHC detected in | mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IF | See 1 publications below |
Product Information
67717-1-Ig targets NMDAR1/GRIN1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, rabbit, chicken samples.
| Tested Reactivity | human, mouse, rat, rabbit, chicken |
| Cited Reactivity | mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag26364 Product name: Recombinant human GRIN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 167-280 aa of NM_000832 Sequence: MVSDDHEGRAAQKRLETLLEERESKAEKVLQFDPGTKNVTALLMEAKELEARVIILSASEDDAATVYRAAAMLNMTGSGYVWLVGEREISGNALRYAPDGILGLQLINGKNESAH 相同性解析による交差性が予測される生物種 |
| Full Name | glutamate receptor, ionotropic, N-methyl D-aspartate 1 |
| Calculated molecular weight | 105 kDa |
| Observed molecular weight | 105-120 kDa |
| GenBank accession number | NM_000832 |
| Gene Symbol | NMDAR1 |
| Gene ID (NCBI) | 2902 |
| RRID | AB_2882906 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q05586 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NMDAR1/GRIN1 antibody 67717-1-Ig | Download protocol |
| IHC protocol for NMDAR1/GRIN1 antibody 67717-1-Ig | Download protocol |
| WB protocol for NMDAR1/GRIN1 antibody 67717-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Exp Brain Res The NR2B-targeted intervention alleviates the neuronal injuries at the sub-acute stage of cerebral ischemia: an exploration of stage-dependent strategy against ischemic insults | ||
Heliyon A peptide from the Japanese encephalitis virus failed to induce the production of anti-N-methyl-d-aspartate receptor antibodies via molecular mimicry in mice | ||
Biomedicines D-Serine May Ameliorate Hippocampal Synaptic Plasticity Impairment Induced by Patients' Anti-N-methyl-D-aspartate Receptor Antibodies in Mice | ||
Biomedicines Impaired Response to Mismatch Novelty in the Li2+-Pilocarpine Rat Model of TLE: Correlation with Hippocampal Monoaminergic Inputs |