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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GRIK5 Polyclonal antibody

GRIK5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28550-1-AP

Synonyms

EAA2, Glutamate receptor KA 2, GRIK2, GRIK5, KA2

Validation Data Gallery

Applications:
  • WB analysis using 28550-1-AP

    WB analysis using 28550-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28550-1-AP (GRIK5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 28550-1-AP

    IHC staining of mouse brain using 28550-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28550-1-AP (GRIK5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 28550-1-AP

    IHC staining of mouse brain using 28550-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28550-1-AP (GRIK5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

28550-1-AP targets GRIK5 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GRIK5 fusion protein Ag29363 相同性解析による交差性が予測される生物種
Full Name glutamate receptor, ionotropic, kainate 5
Calculated molecular weight 109 kDa
Observed molecular weight~110 kDa
GenBank accession numberNM_001301030
Gene symbol GRIK5
Gene ID (NCBI) 2901
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GRIK5 antibody 28550-1-APDownload protocol
IHC protocol for GRIK5 antibody 28550-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols