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Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using GRID1 Polyclonal antibody (13040-1-AP)

    WB analysis of human brain using 13040-1-AP (same clone as 13040-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 13040-1-AP (GRID1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13040-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using GRID1 Polyclonal antibody (13040-1-AP)

    IP experiment of mouse brain using 13040-1-AP (same clone as 13040-1-PBS)

    IP result of anti-GRID1 (IP:13040-1-AP, 4ug; Detection:13040-1-AP 1:300) with mouse brain tissue lysate 5600ug. This data was developed using the same antibody clone with 13040-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using GRID1 Polyclonal antibody (13040-1-AP)

    IHC staining of human gliomas using 13040-1-AP (same clone as 13040-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13040-1-AP (GRID1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13040-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using GRID1 Polyclonal antibody (13040-1-AP)

    IHC staining of human gliomas using 13040-1-AP (same clone as 13040-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13040-1-AP (GRID1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13040-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GRID1 Polyclonal antibody (13040-1-AP)

    IHC staining of mouse brain using 13040-1-AP (same clone as 13040-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13040-1-AP (GRID1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13040-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GRID1 Polyclonal antibody (13040-1-AP)

    IHC staining of mouse brain using 13040-1-AP (same clone as 13040-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13040-1-AP (GRID1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13040-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GRID1 Polyclonal antibody (13040-1-AP)

    IHC staining of mouse brain using 13040-1-AP (same clone as 13040-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13040-1-AP (GRID1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13040-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GRID1 Polyclonal antibody (13040-1-AP)

    IHC staining of mouse brain using 13040-1-AP (same clone as 13040-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13040-1-AP (GRID1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13040-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using GRID1 Polyclonal antibody (13040-1-AP)

    IHC staining of human brain using 13040-1-AP (same clone as 13040-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13040-1-AP (GRID1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13040-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using GRID1 Polyclonal antibody (13040-1-AP)

    IHC staining of human brain using 13040-1-AP (same clone as 13040-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13040-1-AP (GRID1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13040-1-PBS in a different storage buffer formulation.

GRID1 Polyclonal antibody, PBS Only

GRID1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 13040-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

GluD1, GluR delta 1 subunit, GluR delta-1 subunit, Glutamate receptor ionotropic, delta-1, KIAA1220

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13040-1-PBS targets GRID1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3694

Product name: Recombinant human GRID1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 24-348 aa of BC039263

Sequence: IHIGAIFEENAAKDDRVFQLAVSDLSLNDDILQSEKITYSIKVIEANNPFQAVQEACDLMTQGILALVTSTGCASANALQSLTDAMHIPHLFVQRNPGGSPRTACHLNPSPDGEAYTLASRPPVRLNDVMLRLVTELRWQKFVMFYDSEYDIRGLQSFLDQASRLGLDVSLQKVDKNISHVFTSLFTTMKTEELNRYRDTLRRAILLLSPQGAHSFINEAVETNLASKDSHWVFVNEEISDPEILDLVHSALGRMTVVRQIFPSAKDNQKCTRNNHRISSLLCDPQEGYLQMLQISNLYLYDSVLMLANAFHRKLEDRKWHSMAS

相同性解析による交差性が予測される生物種
Full Name glutamate receptor, ionotropic, delta 1
Calculated molecular weight 1009 aa, 112 kDa
Observed molecular weight 100-112 kDa
GenBank accession numberBC039263
Gene Symbol GRID1
Gene ID (NCBI) 2894
RRIDAB_10638439
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9ULK0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

GRID1 (Glutamate Ionotropic Receptor Delta Type Subunit 1) is a protein-coding gene that belongs to the family of ionotropic glutamate receptors. These receptors are crucial for rapid excitatory synaptic transmission and synaptic plasticity in the central nervous system. GRID1 is primarily expressed in various brain regions, including the cortex, hippocampus, amygdala, striatum, thalamus, nucleus accumbens, lateral hooked nucleus, and dorsal raphe nucleus. In the hippocampus, GRID1 is specifically expressed in the granule cells of the dentate gyrus.

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