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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GRIA1 Monoclonal antibody (67642-1-Ig)

    WB analysis using 67642-1-Ig (same clone as 67642-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67642-1-Ig (GRIA1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67642-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig spinal cord tissue using GRIA1 Monoclonal antibody (67642-1-Ig)

    WB analysis of pig spinal cord using 67642-1-Ig (same clone as 67642-1-PBS)

    pig spinal cord tissue were subjected to SDS PAGE followed by western blot with 67642-1-Ig (GRIA1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67642-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig cerebellum tissue using GRIA1 Monoclonal antibody (67642-1-Ig)

    WB analysis of pig cerebellum using 67642-1-Ig (same clone as 67642-1-PBS)

    pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 67642-1-Ig (GRIA1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67642-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using GRIA1 Monoclonal antibody (67642-1-Ig)

    WB analysis of pig brain using 67642-1-Ig (same clone as 67642-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67642-1-Ig (GRIA1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67642-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GRIA1 Monoclonal antibody (67642-1-Ig)

    IHC staining of mouse brain using 67642-1-Ig (same clone as 67642-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67642-1-Ig (GRIA1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67642-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GRIA1 Monoclonal antibody (67642-1-Ig)

    IHC staining of mouse brain using 67642-1-Ig (same clone as 67642-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67642-1-Ig (GRIA1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67642-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using GRIA1 Monoclonal antibody (67642-1-Ig)

    IF Staining of mouse brain using 67642-1-Ig (same clone as 67642-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GRIA1 antibody (67642-1-Ig, Clone: 1F4A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67642-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using GRIA1 Monoclonal antibody (67642-1-Ig)

    IF Staining of mouse brain using 67642-1-Ig (same clone as 67642-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GRIA1 antibody (67642-1-Ig, Clone: 1F4A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67642-1-PBS in a different storage buffer formulation.

GRIA1 Monoclonal antibody, PBS Only

GRIA1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 67642-1-PBS
Clone No.1F4A1

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Pig, Mouse, Rat, Rabbit

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

GluA1, GLUH1, GluR 1, GluR A, GluR K1, GLUR1, GLURA, Glutamate receptor 1, GRIA1, HBGR1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67642-1-PBS targets GRIA1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, Pig, Mouse, Rat, Rabbit samples.

Tested Reactivity Human, Pig, Mouse, Rat, Rabbit
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag28932

Product name: Recombinant human GRIA1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 631-906 aa of BC111734

Sequence: TANLAAFLTVERMVSPIESAEDLAKQTEIAYGTLEAGSTKEFFRRSKIAVFEKMWTYMKSAEPSVFVRTTEEGMIRVRKSKGKYAYLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGIATPKGSALRNPVNLAVLKLNEQGLLDKLKNKWWYDKGECGSGGGDSKDKTSALSLSNVAGVFYILIGGLGLAMLVALIEFCYKSRSESKRMKGFCLIPQQSINEAIRTSTLPRNSGAGASSGGSGENGRVVSHDFPKSMQSIPCMSHSSGMPLGATGL

相同性解析による交差性が予測される生物種
Full Name glutamate receptor, ionotropic, AMPA 1
Calculated molecular weight 906 aa, 102 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC111734
Gene Symbol GRIA1
Gene ID (NCBI) 2890
RRIDAB_2882842
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP42261
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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