Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse cerebellum tissue, rat cerebellum tissue |
| Positive IHC detected in | human brain tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
25012-1-AP targets GRIA1 in WB, IHC, IF, ELISA applications and shows reactivity with human, Rat, Mouse samples.
| Tested Reactivity | human, Rat, Mouse |
| Cited Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21502 Product name: Recombinant human GRIA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 125-249 aa of BC111734 Sequence: LRPELQDALISIIDHYKWQKFVYIYDADRGLSVLQKVLDTAAEKNWQVTAVNILTTTEEGYRMLFQDLEKKKERLVVVDCESERLNAILGQIIKLEKNGIGYHYILANLGFMDIDLNKFKESGAN 相同性解析による交差性が予測される生物種 |
| Full Name | glutamate receptor, ionotropic, AMPA 1 |
| Calculated molecular weight | 906 aa, 102 kDa |
| Observed molecular weight | 100 kDa |
| GenBank accession number | BC111734 |
| Gene Symbol | GRIA1 |
| Gene ID (NCBI) | 2890 |
| RRID | AB_3085767 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P42261 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GRIA1 antibody 25012-1-AP | Download protocol |
| WB protocol for GRIA1 antibody 25012-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Food Chem Toxicol Melatonin attenuates BDE-209-caused spatial memory deficits in juvenile rats through NMDAR-CaMKⅡγ-mediated synapse-to-nucleus signaling | ||
Biomed Chromatogr Revealing the optimal traditional processing methods and its protective effects against febrile seizures of Arisaema cum bile | ||
Cells Treadmill Exercise Facilitates Synaptic Plasticity in APP/PS1 Mice by Regulating Hippocampal AMPAR Activity | ||
Behav Pharmacol Hesperidin produces antidepressant effects by activating AMPA receptor: enhancing synaptic proteins to promote hippocampal neuronal activities | ||
J Ethnopharmacol Iridoids from Gardeniae fructus ameliorates depression by enhancing synaptic plasticity via AMPA receptor-mTOR signaling |
