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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using GREB1 Recombinant antibody (85189-4-RR)

    WB analysis of MCF-7 using 85189-4-RR (same clone as 85189-4-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 85189-4-RR (GREB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85189-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of T-47D cells using GREB1 Recombinant antibody (85189-4-RR)

    WB analysis of T-47D using 85189-4-RR (same clone as 85189-4-PBS)

    T-47D cells were subjected to SDS PAGE followed by western blot with 85189-4-RR (GREB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85189-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85189-4-RR

    Affinity and Kinetic Characterization of 85189-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85189-4-RR against Human GREB1 were performed. The affinity constant is 45.5 nM.

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GREB1 Recombinant antibody, PBS Only

GREB1 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 85189-4-PBS
Clone No.242882D11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

242882D11, GREB1 protein, KIAA0575, Protein GREB1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85189-4-PBS targets GREB1 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GREB1 fusion protein Ag29908 相同性解析による交差性が予測される生物種
Full Name GREB1 protein
Calculated molecular weight 216 kDa
Observed molecular weight 216-240 kDa
GenBank accession numberNM_014668
Gene Symbol GREB1
Gene ID (NCBI) 9687
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ4ZG55
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

GREB1 (Gene regulated by estrogen in breast cancer protein1) encodes a 1949-amino acid protein that contains an N-terminal Zn-binding domain followed by a circularly permuted catalytically inactive superfamily II helicase domain and a C-terminal Fringe-like GT domain. GREB1 is an early estrogen-responsive gene, and its expression is correlated with estrogen levels in breast cancer patients. GREB1 is implicated in hormone-dependent cancers, such as ovarian, prostate cancer and as a risk factor in endometriosis and variations in bone mineral density.