Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells, HEK-293 cells |
| Positive IP detected in | A431 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 6 publications below |
| IHC | See 3 publications below |
| IF | See 1 publications below |
Product Information
10045-1-Ig targets GRB7 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0023 Product name: Recombinant human GRB7 protein Source: e coli.-derived, Unknow Tag: N/A Domain: Gst-KG-Grb7 of BC006535 Sequence: 相同性解析による交差性が予測される生物種 |
| Full Name | growth factor receptor-bound protein 7 |
| Calculated molecular weight | 532 aa, 60 kDa |
| Observed molecular weight | 60-70 kDa |
| GenBank accession number | BC006535 |
| Gene Symbol | GRB7 |
| Gene ID (NCBI) | 2886 |
| RRID | AB_2232601 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q14451 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Grb7 belongs to a family of adapter proteins. This family, including Grb10, Grb7 and Grb 14, contains a praline-rich, a RA, a PH, a BPS and SH2 domain. Grb7 associate with a number of receptors including EGFR and FAK. Functions of Grb7 include mobility because it associates receptors such FAK, and direct evidence from overexpression of this proteins.This antibody recognize the N-terminal of GRB7.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GRB7 antibody 10045-1-Ig | Download protocol |
| IHC protocol for GRB7 antibody 10045-1-Ig | Download protocol |
| IP protocol for GRB7 antibody 10045-1-Ig | Download protocol |
| WB protocol for GRB7 antibody 10045-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Transl Med TCF12-regulated GRB7 facilitates the HER2+ breast cancer progression by activating Notch1 signaling pathway | ||
Front Microbiol GRB7-mediated enhancement of cell malignant characteristics induced by Helicobacter pylori infection | ||
Pharmaceuticals (Basel) GRB7 Plays a Vital Role in Promoting the Progression and Mediating Immune Evasion of Ovarian Cancer
| ||
Int Immunopharmacol ACOT8-mediated palmitate accumulation promotes M1 macrophage polarization in renal ischemia-reperfusion injury via activation of the cGAS-STING pathway. | ||
Front Pharmacol Astragalus propinquus schischkin and Salvia miltiorrhiza bunge promote angiogenesis to treat myocardial ischemia via Ang-1/Tie-2/FAK pathway | ||
Mol Carcinog TRIM36 Inhibits the Development of AOM/DSS-Induced Colitis-Associated Colorectal Cancer by Promoting the Ubiquitination and Degradation of GRB7 |