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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GRASP65,GORASP1 Monoclonal antibody (66651-1-Ig)

    WB analysis using 66651-1-Ig (same clone as 66651-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66651-1-Ig (GRASP65,GORASP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66651-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using GRASP65,GORASP1 Monoclonal antibody (66651-1-Ig)

    WB analysis of HSC-T6 using 66651-1-Ig (same clone as 66651-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66651-1-Ig (GRASP65,GORASP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66651-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using GRASP65,GORASP1 Monoclonal antibody (66651-1-Ig)

    IHC staining of human prostate cancer using 66651-1-Ig (same clone as 66651-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66651-1-Ig (GRASP65,GORASP1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66651-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using GRASP65,GORASP1 Monoclonal antibody (66651-1-Ig)

    IHC staining of human prostate cancer using 66651-1-Ig (same clone as 66651-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66651-1-Ig (GRASP65,GORASP1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66651-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using GRASP65,GORASP1 Monoclonal antibody (66651-1-Ig)

    IHC staining of human stomach cancer using 66651-1-Ig (same clone as 66651-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66651-1-Ig (GRASP65,GORASP1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66651-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using GRASP65,GORASP1 Monoclonal antibody (66651-1-Ig)

    IHC staining of human stomach cancer using 66651-1-Ig (same clone as 66651-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66651-1-Ig (GRASP65,GORASP1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66651-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SK-BR-3 cells using GRASP65,GORASP1 Monoclonal antibody (66651-1-Ig)

    IF Staining of SK-BR-3 using 66651-1-Ig (same clone as 66651-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SK-BR-3 cells using GRASP65,GORASP1 antibody (66651-1-Ig, Clone: 1B2C10 ) at dilution of 1:3876 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66651-1-PBS in a different storage buffer formulation.

GRASP65,GORASP1 Monoclonal antibody, PBS Only

GRASP65,GORASP1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66651-1-PBS
Clone No.1B2C10

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

GORASP1, 1B2C10, Golgi phosphoprotein 5, Golgi reassembly-stacking protein 1, Golgi reassembly-stacking protein of 65 kDa

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66651-1-PBS targets GRASP65,GORASP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag1054

Product name: Recombinant human GRASP65,GORASP1 protein

Source: e coli.-derived, PKG

Tag: GST

Domain: 1-215 aa of BC008928

Sequence: MGLGVSAEQPAGGAEGFHLHGVQENSPAQQAGLEPYFDFIITIGHSRLNKENDTLKALLKANVEKPVKLEVFNMKTMRVREVEVVPSNMWGGQGLLGASVRFCSFRRASEQVWHVLDVEPSSPAALAGLRPYTDYVVGSDQILQESEDFFTLIESHEGKPLKLMVYNSKSDSCRESGMWHWLWVSTPDPNSAPQLPQEATWHPTTFCSTTWCPTT

相同性解析による交差性が予測される生物種
Full Name golgi reassembly stacking protein 1, 65kDa
Calculated molecular weight 46 kDa
Observed molecular weight65-70 kDa
GenBank accession numberBC008928
Gene Symbol GRASP65
Gene ID (NCBI) 64689
RRIDAB_2882008
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ9BQQ3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Golgi reassembly-stacking protein 1 is a protein that in humans is encoded by the GORASP1 gene. The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum.

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