GRAMD1A Polyclonal antibody
GRAMD1A Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, ELISA
Conjugate
Unconjugated
Cat no : 25487-1-AP
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | A549 cells, HEK-293T cells, HeLa cells, SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Sample-dependent, check data in validation data gallery | |
Product Information
25487-1-AP targets GRAMD1A in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GRAMD1A fusion protein Ag22221 相同性解析による交差性が予測される生物種 |
| Full Name | GRAM domain containing 1A |
| Calculated molecular weight | 724 aa, 81 kDa |
| Observed molecular weight | 80-90 kDa |
| GenBank accession number | BC014077 |
| Gene symbol | GRAMD1A |
| Gene ID (NCBI) | 57655 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Protein Aster-A (GRAMD1A) is a cholesterol transporter, which contains unique domains for binding cholesterol and the plasma membrane (PM), thereby serving as a molecular bridge for the transfer of cholesterol from the PM to the endoplasmic reticulum (ER). It was reported that plays a role in autophagy regulation and is required for the biogenesis of the autophagosome (PMID:31222192). It can be detected as 80 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GRAMD1A antibody 25487-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
