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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using GPX5 Polyclonal antibody (18731-1-AP)

    WB analysis of mouse testis using 18731-1-AP (same clone as 18731-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 18731-1-AP (GPX5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18731-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using GPX5 Polyclonal antibody (18731-1-AP)

    WB analysis of mouse liver using 18731-1-AP (same clone as 18731-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 18731-1-AP (GPX5 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18731-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using GPX5 Polyclonal antibody (18731-1-AP)

    WB analysis of human testis using 18731-1-AP (same clone as 18731-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 18731-1-AP (GPX5 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18731-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using GPX5 Polyclonal antibody (18731-1-AP)

    WB analysis of PC-3 using 18731-1-AP (same clone as 18731-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 18731-1-AP (GPX5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18731-1-PBS in a different storage buffer formulation.

GPX5 Polyclonal antibody, PBS Only

GPX5 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 18731-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

EC:1.11.1.9, EGLP, Epididymis-specific glutathione peroxidase-like protein, GPx 5, GPx-5

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18731-1-PBS targets GPX5 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name glutathione peroxidase 5 (epididymal androgen-related protein)
Calculated molecular weight 25 kDa
Observed molecular weight 22-28 kDa
GenBank accession numberNM_001509
Gene Symbol GPX5
Gene ID (NCBI) 2880
RRIDAB_10596913
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO75715
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

GPX5 belongs to the glutathione peroxidase family. GPX5 protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. Catalytic the reaction: 2 glutathione + H2O2 = glutathione disulfide + 2 H2O. GPX5 constitute a glutathionine peroxidase-like protective system against peroxide damage in sperm membrane lipids. GPX5 has a calculated molecular mass of 25 kDa and apparent molecular mass of 22-28 kDa due to the cleavage of signal peptide and glycosylation.

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