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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GPX1 Polyclonal antibody (29329-1-AP)

    WB analysis using 29329-1-AP (same clone as 29329-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29329-1-AP (GPX1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29329-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GPX1 Polyclonal antibody (29329-1-AP)

    WB analysis using 29329-1-AP (same clone as 29329-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29329-1-AP (GPX1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29329-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GPX1 Polyclonal antibody (29329-1-AP)

    WB analysis using 29329-1-AP (same clone as 29329-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29329-1-AP (GPX1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29329-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

GPX1 Polyclonal antibody, PBS Only

GPX1 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 29329-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29329-1-PBS targets GPX1 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag31101

Product name: Recombinant human GPX1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 102-203 aa of BC000742

Sequence: GGGFEPNFMLFEKCEVNGAGAHPLFAFLREALPAPSDDATALMTDPKLITWSPVCRNDVAWNFEKFLVGPDGVPLRRYSRRFQTIDIEPDIEALLSQGPSCA

相同性解析による交差性が予測される生物種
Full Name glutathione peroxidase 1
Calculated molecular weight 22 kDa
Observed molecular weight22 kDa
GenBank accession numberBC000742
Gene Symbol GPX1
Gene ID (NCBI) 2876
RRIDAB_2918283
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP07203
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Gpx protein, known as selenoprotein, consists selenium in its catalytic site. Gpx1 is an abundant isoform, which involves in scavenging endogenous ROS using electron provided by reduced glutathione and ubiquitously expressed the cytoplasm and mitochondria of all cell types. (PMID: 30632027)

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