Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 26798-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 26798-1-AP (GPSM2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 26798-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 26798-1-AP (GPSM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293T using 26798-1-AP
HEK-293T cells were subjected to SDS PAGE followed by western blot with 26798-1-AP (GPSM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 26798-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 26798-1-AP (GPSM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 26798-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 26798-1-AP (GPSM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 26798-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 26798-1-AP (GPSM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human placenta using 26798-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26798-1-AP (GPSM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 26798-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26798-1-AP (GPSM2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 26798-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26798-1-AP (GPSM2 antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human liver using 26798-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26798-1-AP (GPSM2 antibody) at dilution of 1:200 (under 40x lens).
IF Staining of MCF-7 using 26798-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 26798-1-AP (GPSM2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

GPSM2 Polyclonal antibody

GPSM2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 26798-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

GPSM2, LGN, Mosaic protein LGN, Pins

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, mouse heart tissue, HEK-293T cells, mouse liver tissue, HEK-293 cells
Positive IHC detected in	human placenta tissue, human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IHC	See 1 publications below

Product Information

26798-1-AP targets GPSM2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag25195 Product name: Recombinant human GPSM2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 559-600 aa of BC027732 Sequence: FSNLPGLRLTQNSQSVLSHLMTNDNKEADEDFFDILVKCQGS 相同性解析による交差性が予測される生物種
Full Name	G-protein signaling modulator 2 (AGS3-like, C. elegans)
Calculated molecular weight	75 kDa
Observed molecular weight	70-77 kDa
GenBank accession number	BC027732
Gene Symbol	GPSM2
Gene ID (NCBI)	29899
RRID	AB_2880640
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P81274
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GPSM2 belongs to a family of proteins that modulate activation of G proteins. GPSM2 assists in the exchange of guanine nucleotides, and allows extracellular signals to be transmitted to cells via cell surface, and ultimately plays a key role in the activation of G-proteins. Therefore, GPSM2 is a critical factor for the stability of cell division. Some recent studies have shown that GPSM2 messenger RNA (mRNA) is overexpressed and plays a positive role in the development of certain tumors, such as liver cancer, pancreatic cancer, breast cancer. It also plays a role in neuroblast division and in the development of normal hearing. Mutations in GPSM2 are associated with autosomal recessive nonsyndromic deafness (DFNB82), which is a form of non-syndromic deafness characterized by prelingual, bilateral, severe, sensorineural hearing loss.

Protocols

Product Specific Protocols
IF protocol for GPSM2 antibody 26798-1-AP	Download protocol
IHC protocol for GPSM2 antibody 26798-1-AP	Download protocol
WB protocol for GPSM2 antibody 26798-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	J Cell Mol Med Comprehensive Analysis of GPSM2: From Pan-Cancer Analysis to Experimental Validation Authors - Chunjiao Yang View Article
human	IHC	Nan Fang Yi Ke Da Xue Xue Bao [GPSM2 is highly expressed in gastric cancer to affect patient prognosis by promoting tumor cell proliferation] Authors - Xue Song View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 26798-1-AP

WB analysis of mouse heart using 26798-1-AP

WB analysis of HEK-293T using 26798-1-AP

WB analysis of mouse liver using 26798-1-AP

WB analysis of HeLa using 26798-1-AP