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  • Featured Product
  • KD/KO Validated

GPRC5A,RAI3 Polyclonal antibody

GPRC5A,RAI3 Polyclonal Antibody for WB, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, FC, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10309-1-AP

Synonyms

GPCR5A, GPRC5A, GPRC5A,RAI3, RAI3, RAIG 1, RAIG1

Validation Data Gallery

Applications:
  • WB analysis using 10309-1-AP

    WB analysis using 10309-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10309-1-AP (GPRC5A,RAI3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 10309-1-AP

    WB analysis of MCF-7 using 10309-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 10309-1-AP (GPRC5A,RAI3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 10309-1-AP

    IHC staining of human lung cancer using 10309-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10309-1-AP (GPRC5A,RAI3 Antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human lung cancer using 10309-1-AP

    IHC staining of human lung cancer using 10309-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10309-1-AP (GPRC5A,RAI3 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human breast cancer using 10309-1-AP

    IHC staining of human breast cancer using 10309-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10309-1-AP (GPRC5A,RAI3 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human breast cancer using 10309-1-AP

    IHC staining of human breast cancer using 10309-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10309-1-AP (GPRC5A,RAI3 Antibody) at dilution of 1:200 (under 40x lens).

  • FC experiment of MCF-7 using 10309-1-AP

    FC experiment of MCF-7 using 10309-1-AP

    1X10^6 MCF-7 cells were stained with 0.2ug GPRC5A,RAI3 antibody (10309-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Tested Applications

Positive WB detected inA375 cells, MCF-7 cells, HeLa cells, RAW 264.7 cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

10309-1-AP targets GPRC5A,RAI3 in WB, FC, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, goat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPRC5A,RAI3 fusion protein Ag0311 相同性解析による交差性が予測される生物種
Full Name G protein-coupled receptor, family C, group 5, member A
Calculated molecular weight 40 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC003665
Gene symbol GPRC5A
Gene ID (NCBI) 9052
RRIDAB_2877732
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GPRC5A, also named as GPCR5A, RAI3 and RAIG1, belongs to the G-protein coupled receptor 3 family. It is an orphan G-protein coupled receptor (GPCR) that has been associated with malignancy and may play a role in the proliferation of breast cancer cells. GPRC5A is a potential molecular target for treatment of breast cancers. It is suppressed in some human lung carcinoma cells. (PMID:15788639, 20563252, 19552806 )

Protocols

Product Specific Protocols
WB protocol for GPRC5A,RAI3 antibody 10309-1-APDownload protocol
IHC protocol for GPRC5A,RAI3 antibody 10309-1-APDownload protocol
FC protocol for GPRC5A,RAI3 antibody 10309-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
goatIHC

Cell Prolif

Spatially multicellular variability of intervertebral disc degeneration by comparative single-cell analysis

Authors - Peng Lin
View Article
humanWB

Sci Total Environ

GPRC5A reduction contributes to pollutant benzo[a]pyrene injury via aggravating murine fibrosis, leading to poor prognosis of IIP patients.

Authors - Ziling Huang
  • KO Validated
View Article
humanWB

Oncol Lett

Clinical significance of reduced GPRC5A expression in surgically resected non-small cell lung cancer.

Authors - Er Jin
View Article
humanWB

Exp Lung Res

GPRC5a suppresses the proliferation of non-small cell lung cancer under wild type p53 background.

Authors - Jin Er
View Article

Mol Med Rep

Lung cancer suppressor gene GPRC5A mediates p53 activity in non‑small cell lung cancer cells in vitro.

Authors - Er Jin
View Article
humanWB

Stem Cell Res Ther

TMT-based quantitative proteomic analysis of spheroid cells of endometrial cancer possessing cancer stem cell properties

Authors - Mingzhu Cao
View Article