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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GPR81 Polyclonal antibody (20146-1-AP)

    WB analysis using 20146-1-AP (same clone as 20146-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20146-1-AP (GPR81 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20146-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using GPR81 Polyclonal antibody (20146-1-AP)

    IHC staining of mouse pancreas using 20146-1-AP (same clone as 20146-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 20146-1-AP (GPR81 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20146-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using GPR81 Polyclonal antibody (20146-1-AP)

    IHC staining of mouse pancreas using 20146-1-AP (same clone as 20146-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 20146-1-AP (GPR81 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20146-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using GPR81 Polyclonal antibody (20146-1-AP)

    IHC staining of human colon using 20146-1-AP (same clone as 20146-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20146-1-AP (GPR81 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20146-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using GPR81 Polyclonal antibody (20146-1-AP)

    IHC staining of human colon using 20146-1-AP (same clone as 20146-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20146-1-AP (GPR81 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20146-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using GPR81 Polyclonal antibody (20146-1-AP)

    IHC staining of human pancreas using 20146-1-AP (same clone as 20146-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 20146-1-AP (GPR81 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20146-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using GPR81 Polyclonal antibody (20146-1-AP)

    IHC staining of human pancreas using 20146-1-AP (same clone as 20146-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 20146-1-AP (GPR81 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20146-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

GPR81 Polyclonal antibody, PBS Only

GPR81 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 20146-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

FKSG80, G protein coupled receptor 104, G protein coupled receptor 81, GPR104, G-protein coupled receptor 104

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20146-1-PBS targets GPR81 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag13682

Product name: Recombinant human GPR81 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 250-346 aa of BC066881

Sequence: SACDPSVHGALHITLSFTYMNSMLDPLVYYFSSPSFPKFYNKLKICSLKPKQPGHSKTQRPEEMPISNLGRRSCISVANSFQSQSDGQWDPHIVEWH

相同性解析による交差性が予測される生物種
Full Name G protein-coupled receptor 81
Calculated molecular weight 346 aa, 39 kDa
Observed molecular weight39 kDa
GenBank accession numberBC066881
Gene Symbol GPR81
Gene ID (NCBI) 27198
RRIDAB_2878647
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BXC0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

GPR81 (G protein-coupled receptor 81) is one of a large family of GPRs with low affinity for hydroxy-carboxylic acid structure ligands (PMID: 24657625). Lactate functions as a signaling molecule by serving as an agonist for the GPR81, involving both autocrine and paracrine mechanisms (PMID: 31836453). Moreover, A higher molecular mass band (∼60 kDa) is present in the brain and adipose tissue. This band is also strong in the transfected HeLa cells but weak in native HeLa cells and is therefore probably a form of GPR81, possibly a glycosylated form of the receptor (PMID: 23696276).

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