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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse spleen tissue using GPR65 Polyclonal antibody (20306-1-AP)

    IHC staining of mouse spleen using 20306-1-AP (same clone as 20306-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 20306-1-AP (GPR65 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20306-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using GPR65 Polyclonal antibody (20306-1-AP)

    IHC staining of human tonsillitis using 20306-1-AP (same clone as 20306-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20306-1-AP (GPR65 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20306-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using GPR65 Polyclonal antibody (20306-1-AP)

    IHC staining of human tonsillitis using 20306-1-AP (same clone as 20306-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20306-1-AP (GPR65 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20306-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using GPR65 Polyclonal antibody (20306-1-AP)

    IHC staining of mouse spleen using 20306-1-AP (same clone as 20306-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 20306-1-AP (GPR65 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20306-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of RAW 264.7 cells using GPR65 Polyclonal antibody (20306-1-AP)

    FC experiment of RAW 264.7 using 20306-1-AP (same clone as 20306-1-PBS)

    1x10^6 RAW 264.7 cells were intracellularly stained with 0.5 ug GPR65 Polyclonal antibody (20306-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.5 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 20306-1-PBS in a different storage buffer formulation.

GPR65 Polyclonal antibody, PBS Only

GPR65 Polyclonal Antibody for IHC, Indirect ELISA
Cat No. 20306-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, Indirect ELISA

Synonyms

G protein coupled receptor 65, G-protein coupled receptor 65, hTDAG8, Psychosine receptor, T-cell death-associated gene 8 protein

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20306-1-PBS targets GPR65 in IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag14139

Product name: Recombinant human GPR65 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 238-337 aa of BC035633

Sequence: CFTPFHVMLLIRCILEHAVNFEDHSNSGKRTYTMYRITVALTSLNCVADPILYCFVTETGRYDMWNILKFCTGRCNTSQRQRKRILSVSTKDTMELEVLE

相同性解析による交差性が予測される生物種
Full Name G protein-coupled receptor 65
Calculated molecular weight 337 aa, 39 kDa
GenBank accession numberBC035633
Gene Symbol GPR65
Gene ID (NCBI) 8477
RRIDAB_2878668
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IYL9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

GPR65 (G protein-coupled receptor 65) was defined originally as T-cell death-associated gene 8 (TDAG8), functions as a proton-sensing receptor, and is also sensitive to psychosine (PMID: 35343079). GPR65 promotes adaptation to an acidic environment to enhance cell survival and proliferation, thereby promoting tumor development (PMID: 36852075).

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