Validation Data Gallery
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Tested Applications
| Positive WB detected in | A549 cells, Calu-1 cells, K-562 cells, U-87 MG cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32532-1-AP targets GPR56 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein 相同性解析による交差性が予測される生物種 |
| Full Name | G protein-coupled receptor 56 |
| Calculated molecular weight | 78 kDa |
| Observed molecular weight | 65-70 kDa |
| GenBank accession number | NM_001145771.2 |
| Gene Symbol | GPR56 |
| Gene ID (NCBI) | 9289 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9Y653 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GPR56, also known as ADGRG1, is a member of the adhesion G protein-coupled receptor (aGPCR) family. These receptors are characterized by an extended extracellular region with a GPCR autoproteolysis inducing (GAIN) domain (PMID: 37980704). GPR56 is widely expressed in various tissues, including the liver, muscle, tendon, neural cells, and cytotoxic lymphoid cells in humans. It plays crucial roles in multiple biological processes such as cell guidance, adhesion, and development.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GPR56 antibody 32532-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |