Validation Data Gallery
Tested Applications
| Positive WB detected in | rat brain tissue, mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22149-1-AP targets GPR34 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17742 Product name: Recombinant human GPR34 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 267-381 aa of BC020678 Sequence: NSFIVLIIFTICFVPYHAFRFIYISSQLNVSSCYWKEIVHKTNEIMLVLSSFNSCLDPVMYFLMSSNIRKIMCQLLFRRFQGEPSRSESTSEFKPGYSLHDTSVAVKIQSSSKST 相同性解析による交差性が予測される生物種 |
| Full Name | G protein-coupled receptor 34 |
| Calculated molecular weight | 381 aa, 44 kDa |
| Observed molecular weight | 38 kDa |
| GenBank accession number | BC020678 |
| Gene Symbol | GPR34 |
| Gene ID (NCBI) | 2857 |
| RRID | AB_3742067 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UPC5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
G protein-coupled receptor 34 (GPR34) is a 7-transmembrane orphan receptor, belonging to the GPCR family, which affects multiple biological and physiological functions, such as cell proliferation and motility, immune infiltration, gene transcription. GPR34 is indeed highly expressed on ILC1-like cells in the tumors of patients with colon adenocarcinoma, head and neck cancer, and liver cancer (PMID: 39358444).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GPR34 antibody 22149-1-AP | Download protocol |
| WB protocol for GPR34 antibody 22149-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |