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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GPR34 Polyclonal antibody (22149-1-AP)

    WB analysis using 22149-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22149-1-AP (GPR34 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GPR34 Polyclonal antibody (22149-1-AP)

    IHC staining of mouse brain using 22149-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22149-1-AP (GPR34 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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GPR34 Polyclonal antibody

GPR34 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22149-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

G protein coupled receptor 34, Probable G-protein coupled receptor 34

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inrat brain tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22149-1-AP targets GPR34 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPR34 fusion protein Ag17742 相同性解析による交差性が予測される生物種
Full Name G protein-coupled receptor 34
Calculated molecular weight 381 aa, 44 kDa
Observed molecular weight38 kDa
GenBank accession numberBC020678
Gene Symbol GPR34
Gene ID (NCBI) 2857
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UPC5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

G protein-coupled receptor 34 (GPR34) is a 7-transmembrane orphan receptor, belonging to the GPCR family, which affects multiple biological and physiological functions, such as cell proliferation and motility, immune infiltration, gene transcription.

Protocols

Product Specific Protocols
WB protocol for GPR34 antibody 22149-1-APDownload protocol
IHC protocol for GPR34 antibody 22149-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols