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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GPR183 Recombinant monoclonal antibody (87492-4-RR)

    WB analysis using 87492-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87492-4-RR (GPR183 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using GPR183 Recombinant monoclonal antibody (87492-4-RR)

    IF Staining of Raji using 87492-4-RR

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using GPR183 antibody (87492-4-RR, Clone: 260283A6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of Daudi cells using GPR183 Recombinant monoclonal antibody (87492-4-RR)

    IF Staining of Daudi using 87492-4-RR

    Immunofluorescent analysis of (4% PFA) fixed Daudi cells using GPR183 antibody (87492-4-RR, Clone: 260283A6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

GPR183 Recombinant monoclonal antibody

GPR183 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 87492-4-RR
Clone No.260283A6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

EBI2, GPR183/EBI2, CDw381, EBV-induced G-protein coupled receptor 2, Epstein-Barr virus-induced G-protein coupled receptor 2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inRaji cells, Daudi cells, Fetal human brain tissue
Positive IF/ICC detected inRaji cells, Daudi cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87492-4-RR targets GPR183 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name G protein-coupled receptor 183
Calculated molecular weight 361 aa, 41 kDa
Observed molecular weight50-55 kDa
GenBank accession numberBC020752
Gene Symbol EBI2
Gene ID (NCBI) 1880
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP32249
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

G-protein-coupled receptor 183, also known as Epstein-Barr virus-induced gene 2 (EBI2), was initially identified in B cells and is the most upregulated gene after infection with Epstein-Barr virus (PMID:37353188). GPR183 is known to play a key role in the development of antibody responses in secondary lymphoid organs (PMID:23473835).

Protocols

Product Specific Protocols
IF protocol for GPR183 antibody 87492-4-RRDownload protocol
WB protocol for GPR183 antibody 87492-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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