Validation Data Gallery
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Tested Applications
| Positive WB detected in | Jurkat cells, HeLa cells, mouse lung tissue, Raji cells |
| Positive IP detected in | mouse lung tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
Product Information
12377-1-AP targets GPR183/EBI2 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GPR183/EBI2 fusion protein Ag3035 相同性解析による交差性が予測される生物種 |
| Full Name | G protein-coupled receptor 183 |
| Calculated molecular weight | 361 aa, 41 kDa |
| Observed molecular weight | 55-60 kDa |
| GenBank accession number | BC020752 |
| Gene Symbol | EBI2 |
| Gene ID (NCBI) | 1880 |
| RRID | AB_2114261 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P32249 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
G-protein-coupled receptor 183, also known as Epstein-Barr virusinduced gene 2 (EBI2), was initially identified in B cells and is the most upregulated gene after infection with Epstein-Barr virus (PMID:37353188). GPR183 is known to play a key role in the development of antibody responses in secondary lymphoid organs (PMID:23473835).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GPR183/EBI2 antibody 12377-1-AP | Download protocol |
| IP protocol for GPR183/EBI2 antibody 12377-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Allergy Clin Immunol Chronic rhinosinusitis with nasal polyps is characterized by B-cell inflammation and EBV-induced protein 2 expression. | ||
J Pharmacol Exp Ther 7α,25-Dihydroxycholesterol Suppresses Hepatocellular Steatosis through GPR183/EBI2 in Mouse and Human Hepatocytes. | ||
Neurotox Res Phoenixin-20 Ameliorates Lipopolysaccharide-Induced Activation of Microglial NLRP3 Inflammasome. | ||
Eur J Pharmacol Activation of G-protein-coupled receptor 183 initiates inflammatory pain via macrophage CCL22 secretion | ||
Br J Nutr RNA-seq screening and gene function analysis uncover GPR183 as a key mediator for methionine to stimulate milk synthesis in mouse mammary epithelial cells |