Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16691-1-AP targets GPR180 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10052 Product name: Recombinant human GPR180 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 18-170 aa of BC052243 Sequence: QGSQGKTLRGSFSSTAAQDAQGQRIGHFEFHGDHALLCVRINNIAVAVGKEAKLYLFQAQEWLKLQQSSHGYSCSEKLSKAQLTMTMNQTEHNLTVSQIPSPQTWHVFYADKYTCQDDKENSQVEDIPFEMVLLNPDAEGNPFDHFSAGESGL 相同性解析による交差性が予測される生物種 |
| Full Name | G protein-coupled receptor 180 |
| Calculated molecular weight | 440 aa, 49 kDa |
| Observed molecular weight | 49 kDa |
| GenBank accession number | BC052243 |
| Gene Symbol | GPR180 |
| Gene ID (NCBI) | 160897 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86V85 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GPR180 is an orphan GPCR, also known as the Endothelial Thickness-Related Receptor (ITR), and is a member of the GOLDS domain seven transmembrane helix (GOST) protein family. This receptor was initially discovered to promote the proliferation of vascular smooth muscle cells, leading to subsequent thickening of the vascular intima. GPR180 interacts with CTHRC1 to regulate the TGFβ signaling pathway, influencing energy metabolism and thermogenic function ( PMID: 34880217).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GPR180 antibody 16691-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |