Validation Data Gallery
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Tested Applications
| Positive WB detected in | SH-SY5Y cells, HeLa cells, human brain tissue, Y79 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
Product Information
15254-1-AP targets GPR162 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7011 Product name: Recombinant human GPR162 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 328-588 aa of BC002353 Sequence: ERYRADVRTVWEQCVAIMSEEDGDDDGGCDDYAEGRVCKVRFDANGATGPGSRDPAQVKLLPGRHMLFPPLERVHYLQVPLSRRLSHDETNIFSTPREPGSFLHKWSSSDDIRVLPAQSRALGGPPEYLGQRHRLEDEEDEEEAEGGGLASLRQFLESGVLGSGGGPPRGPGFFREEITTFIDETPLPSPTASPGHSPRRPRPLGLSPRRLSLGSPESRAVGLPLGLSAGRRCSLTGGEESARAWGGSWGPGNPIFPQLTL 相同性解析による交差性が予測される生物種 |
| Full Name | G protein-coupled receptor 162 |
| Calculated molecular weight | 64 kDa |
| Observed molecular weight | 33 kDa, 64 kDa |
| GenBank accession number | BC002353 |
| Gene Symbol | GPR162 |
| Gene ID (NCBI) | 27239 |
| RRID | AB_10665369 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16538 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GPR162 antibody 15254-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
JCI Insight Transient expansion and myofibroblast conversion of adipogenic lineage precursors mediate bone marrow repair after radiation. | ||
Acta Neuropathol Commun Blood-based CNS regionally and neuronally enriched extracellular vesicles carrying pTau217 for Alzheimer's disease diagnosis and differential diagnosis | ||
Bone Marrow adipogenic lineage precursors (MALPs) facilitate bone marrow recovery after chemotherapy |