Validation Data Gallery

Filter: AllWB

WB analysis of SH-SY5Y using 15254-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15254-1-AP (GPR162 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Y79 using 15254-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 15254-1-AP (GPR162 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 15254-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 15254-1-AP (GPR162 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 15254-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 15254-1-AP (GPR162 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15254-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15254-1-AP (GPR162 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

GPR162 Polyclonal antibody

GPR162 Polyclonal Antibody for WB, ELISA

Cat No. 15254-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

A 2, G protein coupled receptor 162, GPR162, GRCA

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in

SH-SY5Y cells, HeLa cells, human brain tissue, Y79 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IHC	See 2 publications below
IF	See 1 publications below

Product Information

15254-1-AP targets GPR162 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7011 Product name: Recombinant human GPR162 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 328-588 aa of BC002353 Sequence: ERYRADVRTVWEQCVAIMSEEDGDDDGGCDDYAEGRVCKVRFDANGATGPGSRDPAQVKLLPGRHMLFPPLERVHYLQVPLSRRLSHDETNIFSTPREPGSFLHKWSSSDDIRVLPAQSRALGGPPEYLGQRHRLEDEEDEEEAEGGGLASLRQFLESGVLGSGGGPPRGPGFFREEITTFIDETPLPSPTASPGHSPRRPRPLGLSPRRLSLGSPESRAVGLPLGLSAGRRCSLTGGEESARAWGGSWGPGNPIFPQLTL 相同性解析による交差性が予測される生物種
Full Name	G protein-coupled receptor 162
Calculated molecular weight	64 kDa
Observed molecular weight	33 kDa, 64 kDa
GenBank accession number	BC002353
Gene Symbol	GPR162
Gene ID (NCBI)	27239
RRID	AB_10665369
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q16538
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for GPR162 antibody 15254-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC	Acta Neuropathol Commun Blood-based CNS regionally and neuronally enriched extracellular vesicles carrying pTau217 for Alzheimer's disease diagnosis and differential diagnosis Authors - Zhen Guo View Article
mouse	IF	Bone Marrow adipogenic lineage precursors (MALPs) facilitate bone marrow recovery after chemotherapy Authors - Huan Wang View Article
	IHC	JCI Insight Transient expansion and myofibroblast conversion of adipogenic lineage precursors mediate bone marrow repair after radiation. Authors - Leilei Zhong View Article

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Target

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Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of SH-SY5Y using 15254-1-AP

WB analysis of Y79 using 15254-1-AP

WB analysis of human brain using 15254-1-AP