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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GPR155 Polyclonal antibody (12659-1-AP)

    WB analysis using 12659-1-AP (same clone as 12659-1-PBS)

    mouse brain lysates were subjected to SDS PAGE followed by western blot with 12659-1-AP (GPR155 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12659-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using GPR155 Polyclonal antibody (12659-1-AP)

    IP experiment of mouse brain using 12659-1-AP (same clone as 12659-1-PBS)

    IP result of anti-GPR155 (IP:12659-1-AP, 4ug; Detection:12659-1-AP 1:300) with mouse brain tissue lysate 3000ug. This data was developed using the same antibody clone with 12659-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using GPR155 Polyclonal antibody (12659-1-AP)

    IHC staining of human brain using 12659-1-AP (same clone as 12659-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12659-1-AP (GPR155 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12659-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using GPR155 Polyclonal antibody (12659-1-AP)

    IHC staining of human brain using 12659-1-AP (same clone as 12659-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12659-1-AP (GPR155 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12659-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GPR155 Polyclonal antibody (12659-1-AP)

    IHC staining of mouse brain using 12659-1-AP (same clone as 12659-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12659-1-AP (GPR155 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12659-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GPR155 Polyclonal antibody (12659-1-AP)

    IHC staining of mouse brain using 12659-1-AP (same clone as 12659-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12659-1-AP (GPR155 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12659-1-PBS in a different storage buffer formulation.

GPR155 Polyclonal antibody, PBS Only

GPR155 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 12659-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12659-1-PBS targets GPR155 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3328

Product name: Recombinant human GPR155 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 540-870 aa of BC028730

Sequence: MCMNQTAQAGGYEGFDQSQSHKVVEPGNTAFEESPAPVNEPELFTSSIPETSCCSCSMGNGELHCPSIEPIANTSTSEPVIPSFEKNNHCVSRCNSQSCILAQEEEQYLQSGDQQLTRHVLLCLLLIIGLFANLYSCLWWLFNQEPGRLYVELQFFCAVFNFGQGFISFGIFGLDKHLIILPFKRRLEFLWNNKDTAENRDSPVSEEIKMTCQQFIHYHRDLCIRNIVKERRCGAKTSAGTFCGCDLVSWLIEVGLASDRGEAVIYGDRLVQGGVIQHITNEYEFRDEYLFYRFLQKSPEQSPPAINANTLQQERYKEIEHSSPPSHSPKT

相同性解析による交差性が予測される生物種
Full Name G protein-coupled receptor 155
Calculated molecular weight 870 aa, 97 kDa
Observed molecular weight 97 kDa, 75 kDa
GenBank accession numberBC028730
Gene Symbol GPR155
Gene ID (NCBI) 151556
RRIDAB_2877869
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z3F1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The G Protein-coupled receptor 155 (GPR155), also known as Lysosomal cholesterol signaling protein (LYCHOS), is a cholesterol receptor on the lysosome. GRR155 plays a role in the cholesterol-sensing lysosomal pathway and couples cholesterol concentration to MTORC1-dependent anabolic signaling (PMID: 36007018). GPR155 may represent a biomarker for diagnosing and predicting hematogenous metastasis of gastric cancer (PMID: 28165032).

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