Validation Data Gallery
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Tested Applications
| Positive WB detected in | Jurkat cells, K-562 cells, MCF-7 cells, human placenta tissue, mouse lung tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83511-1-RR targets GPR105 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | GPR105 fusion protein Ag14105 相同性解析による交差性が予測される生物種 |
| Full Name | purinergic receptor P2Y, G-protein coupled, 14 |
| Calculated molecular weight | 338 aa, 39 kDa |
| Observed molecular weight | 40 kDa |
| GenBank accession number | BC034989 |
| Gene Symbol | P2RY14 |
| Gene ID (NCBI) | 9934 |
| RRID | AB_3671136 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q15391 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GPR105 (P2RY14) is widely expressed throughout many brain regions and peripheral tissues of humans and rodents, and couples to a pertussis toxin-sensitive G protein (PMID: 14559350). GPR105 is also prominently expressed in immune cells, including macrophages, lymphocytes, and neutrophils (PMID: 22778393).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GPR105 antibody 83511-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |