Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse brain tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
Product Information
25247-1-AP targets GPR103 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19048 Product name: Recombinant human QRFPR protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 229-356 aa of BC128133 Sequence: EYDDVTIKMIFAIVQIIGFSNSICNPIVYAFMNENFKKNVSSAVCYCIVNKTFSPAQRHGNSGITMMRKKAKFSLRENPVEETKGEAFSDGNIEVKLCEQTEEKKKLKRHLALFRSELAENSPLDSGH 相同性解析による交差性が予測される生物種 |
| Full Name | pyroglutamylated RFamide peptide receptor |
| Calculated molecular weight | 431 aa, 49 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | BC128133 |
| Gene Symbol | GPR103 |
| Gene ID (NCBI) | 84109 |
| RRID | AB_2879987 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96P65 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GPR103 antibody 25247-1-AP | Download protocol |
| WB protocol for GPR103 antibody 25247-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Neurochem N-glycosylation of the human neuropeptide QRFP receptor (QRFPR) is essential for ligand binding and receptor activation. | ||
J Clin Lab Anal The variations in human orphan G protein-coupled receptor QRFPR affect PI3K-AKT-mTOR signaling.
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